|Title:||High-level Tryptophan Accumulation in Seeds of Transgenic Rice and Its Limited Effects on Agronomic Traits and Seed Metabolitc Profile||Authors:||Kyo Wakasa
|Keywords:||Amlno acids;Anthranilate Synthase;AS;Noncontainment greenhouse;IAA;Isolated field;5MT;OASA1D;Oryza sativa;Ecological and Environmental Biosafety of Transgenic Plants : proceedings of international symposium||Issue Date:||Dec-2006||Publisher:||農業試驗所||Related Publication(s):||農業試驗所特刊第126號||Start page/Pages:||163-190||Source:||Ecological and Environmental Biosafety of Transgenic Plants||Conference:||Proceedings of International Symposium Ecological and Environmental Biosafety of Transgenic Plants||Abstract:||
Metabolic manipulation of plants to improve their nutritional quality is an important goal of plant biotechnology. Expression in rice (Oryza sativa L.) of a transgene (OASA1D) encoding a feedback-insensitive a subunit of rice anthranilate synthase results in the accumulation of tryptophan (Trp) in calli and leaves. It is shown here that the amount of free Trp in the seeds of such plants is increased by about two orders of magnitude compared with that in the seeds of wild-type plants. The total Trp content in the seeds of the transgenic plants was also increased. Two homozygous lines, HWI and HWS, of OASA1D transgenic rice were generated for
High-level Tryptophan Accumulation in Seeds of Transgenic Rice
characterization of agronomic traits and aromatic metabolite profiling of seeds. The marked overproduction of Trp was stable in these lines under field conditions, although spikelet fertility and yield, as well as seed germination ability, were reduced compared with the wild type. These differences in agronomic traits were Small, however, in HWS. In spite of the high Trp content in the seeds of the HW lines, metabolic profiling revealed no substantial changes in the amounts of other phenolic compounds. The amount of indole acetic acid was increased about2- fold in the seeds of the transgenic lines. The establishment and characterization of these OASA1D transgenic lines have thus demonstrated the feasibility of increasing the Trp content in the seeds of rice (or of other crops) as a means of improving its nutritional value for human consumption or animal feed.
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