|Title:||A Novel Strategy for Plant Virus Resistance Using Artificial miRNA||Authors:||Shih-Shun Lin
|Keywords:||Ecological and Environmental Biosafety of Transgenic Plants : proceedings of international symposium||Issue Date:||Dec-2006||Publisher:||農業試驗所||Related Publication(s):||農業試驗所特刊第126號||Start page/Pages:||209-220||Source:||Ecological and Environmental Biosafety of Transgenic Plants||Conference:||Proceedings of International Symposium Ecological and Environmental Biosafety of Transgenic Plants||Abstract:||
Plant micro-RNAs (miRNAs) are 21-nt-long Small RNAs that regulate the abundance of target mRNAs by guiding cleavage at the sequence complementary region. We found that DNA sequences encoding Aiabidopsis pre-miRNAs can be expressed from a 35S promoter in transgenic plants to produce mature miRNAs. Moreover, sequences in pre-miRNAs can be modified to produce artificial miRNAs (amiRNAs) of a pre-determined sequence. Here, we describe a novel strategy to express amiRNA designed to target and degrade offending viral RNAs thereby conferring specific resistance. The amiRNA-mediated approach should have broad applicability for engineering multiple virus resistance in crop plants.
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