|Title:||Improving nitrogen use efficiency by manipulating nitrate remobilization in plants||Authors:||Kuo-En Chen
|Issue Date:||Sep-2020||Publisher:||Nature Publishing Group||Journal Volume:||6||Journal Issue:||9||Start page/Pages:||1126-1135||Source:||Nature Plants||Abstract:||
Increasing nitrogen use efficiency (NUE) is critical to improve crop yield, reduce N fertilizer demand and alleviate environmental pollution. N remobilization is a key component of NUE. The nitrate transporter NRT1.7 is responsible for loading excess nitrate stored in source leaves into phloem and facilitates nitrate allocation to sink leaves. Under N starvation, the nrt1.7 mutant exhibits growth retardation, indicating that NRT1.7-mediated source-to-sink remobilization of stored nitrate is important for sustaining growth in plants. To energize NRT1.7-mediated nitrate recycling, we introduced a hyperactive chimeric nitrate transporter NC4N driven by the NRT1.7 promoter into the nrt1.7 mutant. NRT1.7p::NC4N::3′ transgenic plants accumulated more nitrate in younger leaves, and 15NO3− tracing analysis revealed that more 15N was remobilized into sink tissues. Consistently, transgenic Arabidopsis, tobacco and rice plants showed improved growth or yield. Our study suggests that enhancing source-to-sink nitrate remobilization represents a new strategy for enhancing NUE and crop production.
|Appears in Collections:||SCI期刊|
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