https://scholars.tari.gov.tw/handle/123456789/15923
Title: | 根莖直徑與兩階段連續培養方式對薑組培苗 增殖與生長之影響 | Other Titles: | Effects of Rhizome Diameter and Two-Stage Continuous Culture on Proliferation Rate and Growth of In Vitro Zingiber officinale Plantlets | Authors: | 陳威臣 曹進義 吳姿穎 夏奇鈮 Uei-Chern Chen Chin-Yi Tsao Tzu-Ying Wu Chi-Ni Hsia |
Keywords: | 薑;微體繁殖;組培根莖;液態培養;兩階段連續培養;Zingiber officinale;Micropropagation;In vitro rhizome;Liquid culture;Two-stage continuous culture | Issue Date: | Jun-2021 | Publisher: | 農業試驗所 | Journal Volume: | 70 | Journal Issue: | 2 | Start page/Pages: | 117-128 | Source: | 台灣農業研究 | Abstract: | 本研究以「廣東薑」(Zingiber officinale ‘Guang Dong’) 與「竹薑」(Z. officinale ‘Chu’) 組織培養根莖進行試驗,探討根莖直徑與液、固態兩階段連續培養方式對組培苗增殖與生長之影響。將直徑分別約2、4 及6 mm 根莖接種於含有1.0 mg L-1 苯甲基腺嘌呤 (benzylaminopurine; BA) 與0.1 mg L-1 萘乙酸 (α-naphthalene acetic acid; NAA) 之Murashige & Skoog (MS) 固態培養基培養8 wk,結果顯示「廣東薑」每一根莖可得6.2–6.7 芽,但3 種根莖直徑之間並無顯著差異。The influence of rhizome diameter and liquid-solid two-stage continuous culture on proliferation rate and growth of in vitro Zingiber officinale ‘Guang Dong’ and ‘Chu’ plantlets were investigated in this study. The rhizomes of in vitro plantlets with about 2, 4 and 6 mm rhizome diameter were cultured on a Murashige & Skoog (MS) solid medium supplemented with 1 mg L-1 benzylaminopurine (BA) and 0.1 mg L-1 α-naphthalene acetic acid (NAA) for 8 wk of culture. There were 6.2–6.7 plantlets were induced from per explant of ‘Guang Dong’ rhizome, and no significant difference among three size of rhizomes. |
URI: | https://scholars.tari.gov.tw/handle/123456789/15923 | ISSN: | 0022-4847 | DOI: | 10.6156/JTAR.202106_70(2).0004 |
Appears in Collections: | 1.台灣農業研究(1950~迄今) |
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70-2-4.pdf | 1.27 MB | Adobe PDF | View/Open |
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