栽培稻臺稉8號帶抗稻熱病基因Pik-KU之探討

Abstract

栽培稻臺稉8號於1992年由臺南區農業改良場推出，在臺灣部分地區對稻熱病呈現抗病。為探討其抗性來源，本研究以臺稉8號與感病水稻麗江新團黑穀雜交所得之325個F2子代，透過接種本土稻熱病菌菌株13TN-HB1-3分析抗性表現型，並針對主要抗稻熱病基因座Pi2/9、Pik、Pita與Ptr（Pita-2），以四組cleaved amplified polymorphic sequences（CAPS）分子標誌進行基因型鑑定與連鎖分析，發現臺稉8號之抗性與Pik顯著相關。The rice cultivar Taikeng 8, released by Tainan District Agricultural Research and Extension Station in 1992, has exhibited resistance to rice blast in some areas in Taiwan, respectively. To identify the resistance source, a population of 325 F2 progeny derived from the cross of Taikeng 8 and a susceptible cultivar Lijianxintuanheigu (LTH) was developed and used for linkage analysis. The F2 plants were inoculated with a local Magnaporthe oryzae isolate 13TN-HB1-3, and genotyped with four cleaved amplified polymorphic sequences (CAPS) markers targeting the major blast resistance genes Pi2/9, Pik, Pita, and Ptr (Pita-2).