https://scholars.tari.gov.tw/handle/123456789/16520
Title: | An immunochromatographic assay utilizing magnetic nanoparticles to detect major peanut allergen Ara h 1 in processed foods | Authors: | Hsin-Yi Yin Yi-Ting Li Wen-Che Tsai Hung-Yu Dai Hsiao-Wei Wen |
Keywords: | peanut allergen;Ara h 1;immunomagnetic nanoparticle;lateral flow assay | Issue Date: | May-2022 | Publisher: | Elsevier Sci. Ltd., | Journal Volume: | 375 | Journal Issue: | 1 | Start page/Pages: | 131844 | Source: | Food Chemistry | Abstract: | This study describes an immunomagnetic nanoparticle (IMNP)-based lateral flow assay (LFA) for detecting the major peanut allergen Ara h 1. We developed a clearly specific method in identifying peanut from ten other seeds and nuts, and a good visual limit of detection (vLOD) of 0.01 mu g/mL Ara h 1 in PBS. PBS that contains 1 M NaCl and 2% Tween 20 was determined to be the optimal extraction buffer for isolating Ara h 1 from cookie, milk and chocolate with vLOD values of 0.5 mu g/g, 0.5 mu g/mL, and 1 mu g/g, respectively. Forty two processed foods were simultaneously analyzed using this method and an AOAC-approved ELISA kit. The specificity and sensitivity of this assay were thus determined to be 100 and 95%, respectively. This new IMNP-based LFA has potential as a rapid tool for screening processed foods for Ara h 1 residues. |
URI: | https://www.sciencedirect.com/science/article/pii/S0308814621028508?via%3Dihub https://scholars.tari.gov.tw/handle/123456789/16520 |
ISSN: | 0308-8146 | DOI: | 10.1016/j.foodchem.2021.131844 |
Appears in Collections: | SCI期刊 |
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