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Please use this identifier to cite or link to this item: https://scholars.tari.gov.tw/handle/123456789/16520
Title: An immunochromatographic assay utilizing magnetic nanoparticles to detect major peanut allergen Ara h 1 in processed foods
Authors: Hsin-Yi Yin
Yi-Ting Li
Wen-Che Tsai
Hung-Yu Dai 
Hsiao-Wei Wen
Keywords: peanut allergen;Ara h 1;immunomagnetic nanoparticle;lateral flow assay
Issue Date: May-2022
Publisher: Elsevier Sci. Ltd.,
Journal Volume: 375
Journal Issue: 1
Start page/Pages: 131844
Source: Food Chemistry 
Abstract: 
This study describes an immunomagnetic nanoparticle (IMNP)-based lateral flow assay (LFA) for detecting the major peanut allergen Ara h 1. We developed a clearly specific method in identifying peanut from ten other seeds and nuts, and a good visual limit of detection (vLOD) of 0.01 mu g/mL Ara h 1 in PBS. PBS that contains 1 M NaCl and 2% Tween 20 was determined to be the optimal extraction buffer for isolating Ara h 1 from cookie, milk and chocolate with vLOD values of 0.5 mu g/g, 0.5 mu g/mL, and 1 mu g/g, respectively. Forty two processed foods were simultaneously analyzed using this method and an AOAC-approved ELISA kit. The specificity and sensitivity of this assay were thus determined to be 100 and 95%, respectively. This new IMNP-based LFA has potential as a rapid tool for screening processed foods for Ara h 1 residues.
URI: https://www.sciencedirect.com/science/article/pii/S0308814621028508?via%3Dihub
https://scholars.tari.gov.tw/handle/123456789/16520
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2021.131844
Appears in Collections:SCI期刊

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