羽毛分解菌應用於微生物肥料之開發研究

Abstract

本研究篩選具溶磷能力之羽毛分解菌株，經16S rDNA 序列分析其菌株 TCC-2 及 TC4-1C屬於節桿菌屬 (Genus Arthrobacter)，而菌株 PiA 則屬於巨大芽孢桿菌 Bacillus megaterium。菌株 TCC-2、 TC4-1C 及 PiA 經培養於 1% 羽毛之礦物鹽培養基 (BHF medium)至第 4 天，其羽毛分解率分別為 65.3% 、 62.9% 及 44.3%，且羽毛分解液之 pH 、 EC 、銨離子及全氮含量皆明顯提 升。篩選之菌株具有溶解磷礦石粉與磷酸三鈣之能力，其中以菌株 PiA 溶解磷酸三鈣能力較佳 (71.3 mg/L ，培養 4 天)，而菌株 TC4 1C 溶解磷礦石粉能力較佳 (5.04 mg/L ，培養4 天 。菌株 TCC-2 及 TC4-1C 可於 3% 氯化鈉濃度下生長，其中菌株 TC4-1C 可於 1% 台肥即溶 5 號 (10-20-20) 中有效分解羽毛，具有應用生成高養分有機液肥之潛力。篩選之 3 菌株皆可於磷礦粉及草木灰之培養基中分解羽毛，可應用於有機農業之養分補充。 The dual function bacteria possessed feather degradation and phosphate solubilization were isolated as Arthrobacter sp. TCC-2, Arthrobacter sp. TC4-1C and Bacillus megaterium PiA by 16S rDNA sequence analysis. The feather degradation rate by incubation of isolates TCC-2, TC4-1C and PiA in the BHF medium at the fourth day were 65.3%, 62.9% and 44.3% respectively and the pH, EC, NH4+ and total-N in the feather hydrolysates were significantly increased compared with no inoculated ones. Among the three phosphate-solubilizing bacteria, the isolate PiA showed higher phosphate solubilization ability from the calcium phosphate (71.3 mg/L after incubated 4 days) and the isolate TC4-1C showed higher phosphate solubilization ability from the rock phosphate (5.04 mg/L after incubated 4 days). Isolates TCC-2 and TC4-1C can grow in the 3% NaCl nutrient broth medium and the isolate TC4-1C can efficiently degrade feather in 1% instant waster solube fertilizer #5 (10-20-20) medium, showed TC4-1C can be used to produce high nutrient contents of organic liquid fertilizer. The feather also can be degraded by the three isolates in rock phosphate and plant ash medium for applying in the nutrient amendment in the organic farming system.