利用RAPD評估綠豆種原之變異

Abstract

本研究為瞭解綠豆種原之變異，自國家作物種原中心（NPGRC）逄機抽取97個參試材料及利栽培種台南5號做為對照，進行逢機增殖多型性分析（RAPD），並利用變數分析，藉以瞭解數量性狀（5個營養性狀、4個結莢性狀、4個種子狀）在北部春、夏（逆、順境）期作的變異性形。利用14個逢機引子檢測92個參試種原能產生20種性分子標記，此標記將可提供種原判別的資訊，其中6個可提供為核心標記。經RAPD分析後，根據Nei和Li的遺傳相似度估算出一數值矩陣，進行群聚分析可成二群。依分群結果進行逐步複迴歸分析，發現其影響產量主、次因子與數量性狀的結果一致。 The purpose of this study was to estimate the variation in mungbean germplasm. Ninty-seven accessions were randomly sampled from the National Plant Genetic Resources Center (NPGRC) while the local cultivar Tainan 5 (TN5) was included as a check. Randomly amplified polymorphic DNA (RAPD) was investigated in this study. Multivariate analysis was used to estimate the variation in quantitative characters (five vegetative traits, four pod traits, four seed traits) in the spring and summer crops of 1998 at Taipei. The 92 accessions were amplified by RAPD and 14 primers were used. Twenty polymorphic molecular markers were produced, which are a potential source of germplasm information. Six of the 20 markers were core primers for mungbean germplasm. After RAPD analysis, Nei and Li’s genetic similarity formula was used to calculate distances for matrix value. Based on the matrix values, cluster analysis was done. Two different groups (A wide-distance group, B near-distance group) were separated by RAPD markers. Then, according to the results, a stepwise multiple regression analysis of quantitative characters on grain yield was used for group effect. RAPD group results were the same as the use of quantitative characters on grain yield.