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Please use this identifier to cite or link to this item: https://scholars.tari.gov.tw/handle/123456789/1748
Title: The sweet potato sporamin promoter confers high-level phytase expression and improves organic phosphorus acquisition and tuber yield of transgenic potato
Authors: Ya-Fang Hong
Chang-Yeu Liu
Kuo-Joan Cheng
Ai-Ling Hour
Min-Tsair Chan
Tung-Hai Tseng
Kai-Yi Chen
Jei-Fu Shaw
Su-May Yu
Keywords: Sporamin promoter;Phytase;Potato;Sweet potato;Feed additive;Tuber yield and size
Issue Date: Jul-2008
Publisher: Springer
Journal Volume: 67
Journal Issue: 4
Start page/Pages: 347-361
Source: Plant Molecular Biology 
Abstract: 
The sweet potato sporamin promoter was used to control the expression in transgenic potato of the E. coli appA gene, which encodes a bifunctional enzyme exhibiting both acid phosphatase and phytase activities. The sporamin promoter was highly active in leaves, stems and different size tubers of transgenic potato, with levels of phytase expression ranging from 3.8 to 7.4% of total soluble proteins. Phytase expression levels in transgenic potato tubers were stable over several cycles of propagation. Field tests showed that tuber size, number and yield increased in transgenic potato. Improved phosphorus (P) acquisition when phytate was provided as a sole P source and enhanced microtuber formation in cultured transgenic potato seedlings when phytate was provided as an additional P source were observed, which may account for the increase in leaf chloroplast accumulation (important for photosynthesis) and tuber yield of field-grown transgenic potato supplemented with organic fertilizers. Animal feeding tests indicated that the potato-produced phytase supplement was as effective as a commercially available microbial phytase in increasing the availability of phytate-P to weanling pigs. This study demonstrates that the sporamin promoter can effectively direct high-level recombinant protein expression in potato tubers. Moreover, overexpression of phytase in transgenic potato not only offers an ideal feed additive for improving phytate-P digestibility in monogastric animals but also improves tuber yield, enhances P acquisition from organic fertilizers, and has a potential for phytoremediation.
URI: https://scholars.tari.gov.tw/handle/123456789/1748
https://link.springer.com/article/10.1007%2Fs11103-008-9324-6
ISSN: 0167-4412
DOI: 10.1007/s11103-008-9324-6
Appears in Collections:SCI期刊

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