應用直接競爭型酵素免疫分析法檢測農產品中芬普尼、亞滅培、達滅芬、得克利之殘留

Abstract

為評估農藥免疫檢測快篩法之農藥把關效能，應用直接競爭型酵素免疫分析 (direct competitive enzymelinked immunosorbent assay; dcELISA) 商品檢測農產品中之芬普尼、亞滅培、達滅芬、得克利殘留。此4 種農藥的dcELISA 可檢測範圍與檢測極限分別為0.5–100 ng mL-1 與0.23–0.52 ng mL-1。小松菜、胡蘿蔔、綠茶、南瓜、山藥切碎後各外加芬普尼、亞滅培、達滅芬及得克利50 ng mL-1，經萃取、稀釋後以dcELISA 檢測農藥，此4 種農藥之回收率各為73.1–123.6%、61.7–111.1%、72.5–102.3%、67.9–113.9%。顯示農藥免疫檢測之靈敏度高，農藥回收率良好，由檢體萃取至結果判讀僅需1 h，未來可應用在農產品高風險農藥之快篩檢測。 In order to evaluate the rapid screening efficiency of pesticide immunoassay, a commercial direct competitive enzyme-linked immunosorbent assay (dcELISA) was conducted to detect the residues of fipronil, acetamiprid, dimethomorph, and tebuconazole in agricultural samples. The detectable range and the detection limit of these four pesticides by this dcELISA kit were 0.5–100 ng mL-1 and 0.23–0.52 ng mL-1, respectively. Agricultural samples such as komatsuna, carrots, green tea, pumpkin, and yam were chopped and each added with fipronil, acetamiprid, dimethomorph, and tebuconazole to 50 ng mL-1. After extraction and dilution, residual pesticides were detected by dcELISA. The recovery rates of these four pesticides were 73.1–123.6%, 61.7–111.1%, 72.5–102.3% and 67.9–113.9%, respectively, which indicated immunoassay has high sensitivity and a good recovery rate. It takes only one hour from sample extraction to result interpretation, and is adequate for rapid screening of pesticide residues for agricultural products with high risk or known pesticide application records.