https://scholars.tari.gov.tw/handle/123456789/18106| Title: | 以滾環式擴增法簡化雙生病毒感染性選殖株構築流程之研究 | Other Titles: | A simplified procedure for the construction of infectious clones of geminviruses | Authors: | 吳佳穎 廖吉彥 賴儀瑾 蔡欣孜 胡仲祺 Chia-Ying Wu Jye-Yann Liao Yi-Chin Lai Hsin-Tzu Tsai Chung-Chi Hu |
Keywords: | geminiviruses;滾環式擴增法;感染性選殖株;geminiviruses;infectious clone;limited restriction enzyme digestion;rolling circle amplification | Issue Date: | Oct-2007 | Publisher: | 行政院農業委員會動植物防疫檢疫局、國立中興大學農資學院植物病理學系 | Start page/Pages: | 257-278 | Source: | 2007植物蟲媒病害與防治研討會專刊 | Conference: | 2007植物蟲媒病害與防治研討會 | Abstract: | 為提高雙生病毒感染性選殖株的感染效率，在先前的研究中多須採用(部分)多套體的構築方式，以多重步驟的限制酵素裁切、黏合、與再選殖，方能完成構築。 To achieve high infection efficiency, most infectious clones of geminiviruses in earlier studies consist of (partial) tandem repeats of viral genomes in the vectors, which usually involve tedious, multi-step assemblies of genomes fragments in the construction process. |
URI: | https://scholars.tari.gov.tw/handle/123456789/18106 | ISBN: | 978-986-01-1351-8 |
| Appears in Collections: | 植物病理組 |
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