https://scholars.tari.gov.tw/handle/123456789/18109| Title: | 玉米花葯培養單倍體形成之研究 | Other Titles: | Factors Affecting Haploid Plant Regeneration from Maize Anther culture | Authors: | 蔡新聲 Hsin- Sheng Tsay |
Keywords: | 玉米;花葯培養;單倍體;胚狀體;anther culture;haploid plant;maize;regeneration | Issue Date: | 25-Apr-1989 | Publisher: | 中央研究院植物研究所 | Related Publication(s): | 中央研究院植物研究所專刊第七號 | Start page/Pages: | 157-165 | Source: | 玉米之生理遺傳與育種 | Conference: | 玉米生理遺傳與育種研討會 | Abstract: | 以CH-13單交種玉米為材料，檢討花葯培養誘導單倍體形成之培養基及低溫處理條件，結果發現單核期的花葯經9℃處理6-9天效果最好；培養基中蔗糖濃度在3、6、9、12及15%之受測範圍，以9%最適合花葯胚狀體的誘導；植物生長調節劑及活性碳不能提高胚狀體的誘導率，但活性碳可增加每個花葯形成胚狀體的數目。 Anther from CH-13 Zea mays plants were cultured in order to optimize the pretreatment and culture conditions. A cold pretreatment of 6 to 9 days at 9℃ was found to be optimal while 9% sucrose in the culture medium was better than 3,6,12 or 15%. |
URI: | https://scholars.tari.gov.tw/handle/123456789/18109 |
| Appears in Collections: | (2)遺傳資源及生物技術組 |
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