Cultivation of Vegetative Segregants Derived from a Monosporous Culture of Cultivated Mushroom, Agaricus bisporus

Abstract

KLIGMAN (1942) found no differences in cytology and fertility among twelve cultures presumedly derived from single secondary spores of a fertile monosporous race of A. bisporus. IVANOVICH (1965) reported that vegetative segregation in A. bisporus did occur under specific growing conditions in liquid culture. However, PENG and Wu (1972) found vegetative segregation did occur in fertile monosporous culture without in a biculture of two strains showing different inheritance characteristics as described by IVANOVICH (1965). They also stated that two kinds of vegetative segregants with a different colony type could be obtained from mycelial fragments or secondary spores of a monosporous culture. The purpose of this study was to test the fertility of these two kinds of vegetative segregants.