https://scholars.tari.gov.tw/handle/123456789/23056
Title: | JOURNAL OF THE AGRICULTURAL ASSOCIATION OF CHINA | Authors: | CHEN, YH LIU, LF LAI, KL |
Issue Date: | Mar-1990 | Publisher: | Agricultural Association of China | Journal Issue: | 149 | Start page/Pages: | 1-16 | Source: | 中華農學會報 = Journal of the Agricultural Association of China | Abstract: | This expeirment was conducted to study the isolation of rice protoplasts. The results obtained are summarized as follows: 1. Suspension cultured rice cells were used as materials for protoplast isolation. Cell cultured for 5 to 7 days after subculture were suitable for protoplast isolation. Protoplasts were isolated by enzymatic method, that is cells were mixed with enzyme solution containing 4% cellulase R-10, 0.5% macerozyme R-10 and 0.5 M mannitol, and then incubated at 28.degree. C for 5 hrs. The yield of protoplasts was bout 1 .times. 106 protoplasts/ml cells. 2. Changing the suspension medium with fresh medium every other day for three times will promote the growth of suspension cells, increase the percentage of small cell clumps and the protoplast yield. 3. Proper amounts of serine, glycine or methionine added to the enzyme solution are helpful to the isolation of protoplasts. 4. It is suggested that the conditions of suspension cells, enzyme and osmoticum concentration, and the kind of amino acid can influence the isolation of protoplasts. It is necessary to find the proper methods of isolation that could produce more protoplasts for the future experiments. |
URI: | https://scholars.tari.gov.tw/handle/123456789/23056 | ISSN: | 0578-1434 |
Appears in Collections: | SCI期刊 |
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