|Title:||電泳技術在柑橘育種上之應用||Other Titles:||Application of Electrophoresis in Citrus Breeding||Authors:||吳明哲
|Keywords:||柑橘;電泳;同功異構酶;育種;Citrus;electrophoresis;isozymes;breeding||Issue Date:||Dec-1989||Publisher:||農業試驗所||Start page/Pages:||44-49||Source:||農業試驗所特刊第27號||Conference:||柑橘試驗研究成果專題研討會專集||Abstract:||
本研究以本省栽培最重要的柑橘--碰柑、桶柑、柳橙、檸檬、葡萄柚和袖子等的幼嫩葉片或成熟葉片為材料，利用電泳分離技術，探討peroxidase (PRX)、esterase (EST)、superoxide dis-mutase (SOD)、glutamate oxaloacetate transaminase (GOT) shikimic dehydrogenase (ShDH)、malate dehydrogenase (MDH)、及phosphoglucose isomerase (PGI)等七種酵素之同功異構酶基因型之差異，做為將來育種上珠心胚苗與有性胚苗鑑定之依據，以提高育種效率。
Young leaves from plaits of six important citrus species in Taiwan Ponkan (Citrus reticulata), Tankan (C. tankan), Liucheng (C. sinensis), Lemon (C. limon, Hort. var. Eureka), grapefruit (C. paradisi, Hort. var. Ruby) and Pummelo (C. grandis, Hotr. var. Wentan) were used for analyzing isozyme variation by electrophoresis.
Seven enzymes were studied in the experiment, including peroxidase (PRX) esterase (EST), superoxide dismutase (SOD), glutamate oxaloacetate transaminase (GOT), shikimic dehydrogenase (ShDH), malate dehydrogenase (MDH) and phospho glucose isomerase (PGI).
The purpose of this study was to see if any isoenzyme marker can be used to distinguish zygotic from nucellar seedlings in order to increase breeding efficiency.
The results showed that GOT, PRX and PGI are polymorphic among different citrus species. All other four enzymes were monomorphic.
GOT and PRX had been adopted to distinguish zygotic seedlings in a cross between Liucheng and Wentan pummelo.
Isozyme banding of nucellar seedlings had the same patterns with pistil parent (Liucheng).
Hybrid band of PRX isozymes confirmed a true hybrid in certain tested plantlets.
Genetics of isozymes and their application for citrus breeding program was discussed briefly in the report.
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