齒舌蘭輪斑病毒臺灣系統基因序列譯讀與分析

Abstract

Odontoglossum ringspot virus (ORSV) is a member of Tobamovirus genus in the family of Tobamoviridae. It is an important virus infecting orchid plants and occurring worldwide. The purpose of this study is to sequence the genomic regions of ORSV Taiwan strain (ORSV-TW), and to compare its nucleotide and amino acid sequences with that of four reported ORSV strains of various origins, i.e. ORSVS1 (Singapore), ORSV-Cy1 (Japan), ORSV-Cy (Korea) and ORSV-U (America). The full length genomic RNA of ORSV-TW were 6,612 bp (accession number AY571290 . The length of nucleotide sequences for 126/183 kDa RNA-dependent RNA polymerase (RdRp) genes were 3,336 and 4,839 bp and encoded RdRp replicase and readthrough protein, respectively. Genes of movement protein (MP) and coat protein (CP) consisted of 909 and 474 nucleotides and encoded proteins of MW of 33 and 18 kDa, respectively. The nucleotide sequences of 5' and 3' non-translated regions (NTR) were 63 and 414 bp, respectively. The results of comparison between nucleotide and amino acid sequences of ORSV-TW with that of those reported ORSV strains showed more than 96% identity and similarity indicating that they are different strains of the same virus.