|Title:||宜蘭四季蔥感染分蔥潛隱病毒之發生調查與植株感染後之影響評估||Other Titles:||The incidence of Shallot latent virus and its effect on the growth of green onion in Yilan area||Authors:||鄧汀欽
|Keywords:||四季蔥;分蔥潛隱病毒;免疫酵素分析;發生率;封包採樣;種苗;green onion;Shallot latent virus;ELISA;incidence;hierarchical sampling;seedling||Issue Date:||Sep-2003||Publisher:||中華民國植物病理學會||Journal Volume:||12||Journal Issue:||3||Start page/Pages:||191-198||Source:||植物病理學會刊||Abstract:||
利用免疫酵素分析(ELISA)檢測分蔥潛隱病毒(Shallot latent virus, SLV)在感染的蔥株組織中的分佈情形，無論是從葉或花薹取樣，其ELISA反應值均明顯大於對照組，雖然植株間反應值有所差異，但同一株的不同分蘗之問的差異都不顯著，而花薹的反應值則顯著高於葉部，顯示SLV在蔥株不同組織內之濃度雖有高低差別，但其分佈仍屬均勻，取樣調查時不會因採樣部位之不同而獲致不同的結論。調查宜蘭縣三星、員山、五結、壯圍及宜蘭等鄉鎮共81處蔥田，每處蔥田系統性劃分成16個採樣點，每個採樣點進行封包採樣(hierarchical sampling) (8)，再以群體測試(grouping test)進行ELISA，檢測SLV之發生，結果在1296個檢體中，SLV之檢出率平均為60.7%，分另11為63.7%（三星鄉）、64.1%（員山鄉）、90.7%（五結鄉）、70.7%（壯圍鄉）及33.3%（宜蘭市），其中有12處取樣田區其檢出率為0。初次未測得SLV感染之蔥田分別經重複檢測及隔年追蹤調查，仍有2處無發生SLV，可供作健康蔥苗分株採種之用。未經病毒檢定之母株經組織培養後，其成株經檢測其SLV的發生率為16-41%，而經病毒檢定後之母株其組織培養苗在移植於田間二個半月後，僅有21.4％遭到SLV感染。利用健康蔥苗在宜蘭進行進行產量試驗，並以感染SLV的四季蔥作為對照，結果在株高、單株重及莖粗均顯著（P=0.05）較感染SLV的四季蔥為佳。
The distribution of Shallot latent virus (SLV) in infected plant of green onion was revealed by enzyme linked immunosorbent assay (ELISA). Regardless of the tissues sampled from leaves or inflorescence of infected plants, their ELISA values were significantly higher than those in healthy ones. Although the ELISA values in inflorescence tissues were higher than those in leaves in the same infected plant; the differences between tillers were not statistically significant. A total of 81 fields among Sansing, Yuanshan, Wujie, Jhuangwei, and Yilan in Yilan County were surveyed for SLV incidences. Each selected field was systematically divided into 16 sampling plots. Hierarchical sampling method was conducted and grouping test of ELISA was performed to index the incidence of SLV. From 1296 samples tested, the totally average incidence was 60.7%, including 63.7% (Sansing), 64.1% (Yuanshan), 90.7% (Wujie), 70.7% (Jhuangwei) and 33.3% (Yilan). Follow up surveys of SLV infection were conducted in 2001-2002 in 12 fields, which were found with 0% SLV incidences. The result showed that only 2 fields were confirmed maintaining 0% SLV infection and they were thus selected as healthy seedling nurseries. Sixteen to 41% of SLV incidences were found in tissue culture derived green onion plants propagated from non-SLV-tested mother stock, while only 21.6% of incidence was detected in those plants propagated from ELISA screened SLV-free mother stocks 75 days after transplanting into fields. The yield trial in Sansing showed that the growth of healthy plants were significantly better than SLV infected ones in plant height, plant weight, and stem diameter at p=0.05 level.
|Appears in Collections:||植物病理組|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.