菜豆普通嵌紋病毒(BCMV)與胡瓜嵌紋病毒(CMV)的萊豆(Phaseolus limensis)分離株之鑑定與發生

Abstract

Two virus isolates, BCMV-c9 and CMV-Lima, were isolated from diseased lima bean plants (Phaseolus limensis Macf.) cultivated in Taichung and Tainan. After local-lesion isolation on Chenopodium quinoa, BCMV-c9 was identified as a Bean common mosaic virus (BCMV) by ELISA and CMV-Lima was a Cucumber mosaic virus (CMV) tested by SDS-immunodiffusion. For further identification, potyvirus specific primer (Pot1/HRP5) and cucumovirus specific primer (CPTALL-3 / CPTALL-5) were adopted for reverse transcription polymerase chain reaction (RT-PCR). By nucleotide sequence alignment, the CP gene of BCMV-c9 was 89.2% identical to that of Accession number U34972, a BCMV-PSt strain. Phylogenetic analysis also showed that BCMV-c9 was closer to BCMV-PSt strains than to BCMV-BlCM strains. CMV-Lima shared more than 95% of identities with CMV isolates Tfn and NT9 which belong to IB subgroup, 93-94% with isolates Fny and Y which belong to IA subgroup, and only 77-78% with isolates Q and LS which belong to IA subgroup. Consequently, BCMV-c9 is identified as a BCMV-PSt strain and CMV-Lima is a CMV strain that belongs to subgroup IB. From the field survey showed that BCMV was widespread in lima bean cultivated areas, and CMV was occasionally occurred.