Skip navigation
  • 中文
  • English

DSpace CRIS

  • DSpace logo
  • Home
  • Research Outputs
  • Researchers
  • Organizations
  • Projects
  • Explore by
    • Research Outputs
    • Researchers
    • Organizations
    • Projects
  • Academic & Publications
  • Sign in
  • 中文
  • English
  1. DSpace-CRIS at My University
  2. 一、研究單位
  3. 植物病理組
Please use this identifier to cite or link to this item: https://scholars.tari.gov.tw/handle/123456789/4633
Title: 菜豆普通嵌紋病毒(BCMV)與胡瓜嵌紋病毒(CMV)的萊豆(Phaseolus limensis)分離株之鑑定與發生
Other Titles: Occurrence and Identification of Bean Common Mosaic Virus and Cucumber Mosaic Virus Isolated from Lima Bean (Phaseolus limensis)
Authors: 鄧汀欽 
蔡錦慧
蔡惠玲
黃文慶
宋妤
T. C. Deng 
C. H. Tsai
H. L. Tsai
W. C. Huang
Y. Sung
Keywords: BCMV-BlCM;BCMV-PSt;strain;subgroup;phylogenetic analysis;taiwan
Issue Date: Dec-2013
Publisher: 中華植物保護學會
Journal Volume: 55
Journal Issue: 4
Start page/Pages: 105-116
Source: 植物保護學會會刊 
Abstract: 
Two virus isolates, BCMV-c9 and CMV-Lima, were isolated from diseased lima bean plants (Phaseolus limensis Macf.) cultivated in Taichung and Tainan. After local-lesion isolation on Chenopodium quinoa, BCMV-c9 was identified as a Bean common mosaic virus (BCMV) by ELISA and CMV-Lima was a Cucumber mosaic virus (CMV) tested by SDS-immunodiffusion. For further identification, potyvirus specific primer (Pot1/HRP5) and cucumovirus specific primer (CPTALL-3 / CPTALL-5) were adopted for reverse transcription polymerase chain reaction (RT-PCR). By nucleotide sequence alignment, the CP gene of BCMV-c9 was 89.2% identical to that of Accession number U34972, a BCMV-PSt strain. Phylogenetic analysis also showed that BCMV-c9 was closer to BCMV-PSt strains than to BCMV-BlCM strains. CMV-Lima shared more than 95% of identities with CMV isolates Tfn and NT9 which belong to IB subgroup, 93-94% with isolates Fny and Y which belong to IA subgroup, and only 77-78% with isolates Q and LS which belong to IA subgroup. Consequently, BCMV-c9 is identified as a BCMV-PSt strain and CMV-Lima is a CMV strain that belongs to subgroup IB. From the field survey showed that BCMV was widespread in lima bean cultivated areas, and CMV was occasionally occurred.
URI: https://scholars.tari.gov.tw/handle/123456789/4633
ISSN: 0577-750X
DOI: 10.6715/PPB.201312_55(4).0001
Appears in Collections:植物病理組

Files in This Item:
File Description SizeFormat
index.html22.13 kBHTMLView/Open
Show full item record

Page view(s)

32
Last Week
0
Last month
checked on May 22, 2022

Download(s)

10
checked on May 22, 2022

Google ScholarTM

Check

Altmetric

Altmetric

Related Items in TAIR


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Explore by
  • Academic & Publications
  • Research Outputs
  • Researchers
  • Organizations
  • Projects

關於學術典藏系統:收錄本所研究產出,對外展示研究成果,提升學術影響力。

Build with DSpace-CRIS - Extension maintained and optimized by Logo 4SCIENCE Feedback