https://scholars.tari.gov.tw/handle/123456789/4633
Title: | 菜豆普通嵌紋病毒(BCMV)與胡瓜嵌紋病毒(CMV)的萊豆(Phaseolus limensis)分離株之鑑定與發生 | Other Titles: | Occurrence and Identification of Bean Common Mosaic Virus and Cucumber Mosaic Virus Isolated from Lima Bean (Phaseolus limensis) | Authors: | 鄧汀欽 蔡錦慧 蔡惠玲 黃文慶 宋妤 T. C. Deng C. H. Tsai H. L. Tsai W. C. Huang Y. Sung |
Keywords: | BCMV-BlCM;BCMV-PSt;strain;subgroup;phylogenetic analysis;taiwan | Issue Date: | Dec-2013 | Publisher: | 中華植物保護學會 | Journal Volume: | 55 | Journal Issue: | 4 | Start page/Pages: | 105-116 | Source: | 植物保護學會會刊 | Abstract: | Two virus isolates, BCMV-c9 and CMV-Lima, were isolated from diseased lima bean plants (Phaseolus limensis Macf.) cultivated in Taichung and Tainan. After local-lesion isolation on Chenopodium quinoa, BCMV-c9 was identified as a Bean common mosaic virus (BCMV) by ELISA and CMV-Lima was a Cucumber mosaic virus (CMV) tested by SDS-immunodiffusion. For further identification, potyvirus specific primer (Pot1/HRP5) and cucumovirus specific primer (CPTALL-3 / CPTALL-5) were adopted for reverse transcription polymerase chain reaction (RT-PCR). By nucleotide sequence alignment, the CP gene of BCMV-c9 was 89.2% identical to that of Accession number U34972, a BCMV-PSt strain. Phylogenetic analysis also showed that BCMV-c9 was closer to BCMV-PSt strains than to BCMV-BlCM strains. CMV-Lima shared more than 95% of identities with CMV isolates Tfn and NT9 which belong to IB subgroup, 93-94% with isolates Fny and Y which belong to IA subgroup, and only 77-78% with isolates Q and LS which belong to IA subgroup. Consequently, BCMV-c9 is identified as a BCMV-PSt strain and CMV-Lima is a CMV strain that belongs to subgroup IB. From the field survey showed that BCMV was widespread in lima bean cultivated areas, and CMV was occasionally occurred. |
URI: | https://scholars.tari.gov.tw/handle/123456789/4633 | ISSN: | 0577-750X | DOI: | 10.6715/PPB.201312_55(4).0001 |
Appears in Collections: | 植物病理組 |
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