|Title:||胡瓜嵌紋病毒與黑眼豇豆嵌紋病毒複合感染引起的長豇豆皺葉嵌紋病||Other Titles:||Rugose mosaic of asparagus bean caused by dual infection with cucumber mosaic virus and blackeye cowpea mosaic virus||Authors:||張清安
|Keywords:||長豇豆;胡瓜嵌紋病毒;黑眼豇豆嵌紋病毒;相乘作用;病毒間交互作用;asparagus bean;Vigna sesquipedalis;CMV;BICMV;virus interaction;synergism||Issue Date:||Jun-1983||Publisher:||植病保護學會||Journal Volume:||25||Journal Issue:||3||Start page/Pages:||177-190||Source:||植物保護學會會刊||Abstract:||
夏季栽培之長豇豆普遍發生一種嚴重之毒素病，病株葉片出現皺葉嵌紋，分枝減少生育不良，開花及結實率顯著降低，豆莢捲曲變形且有壞疽現象，品質及產量均受嚴重之影響。利用煙草與菜豆可由病株中分離到兩種病毒，其中之一為球形顆粒病毒，根據．其寄主範圍與反應確定為胡瓜嵌紋病毒（Cucumber mosaic virus)，另一為長絲狀病毒，長度約為750 nm左右，依據其寄主範圍與反應以及血清學特性確定為黑眼豇豆嵌紋病毒（Blackeye cowpea mosaic virus）。二者分別感染長豇豆時並不產生皺葉嵌紋病徵，但是將兩病毒混合接種或前後接種，則可產生與田間病株完全相同的皺葉嵌紋病徵。在溫室管理之狀祝下單獨感染任一病毒之病株，其產量與品質與不接種對照之差異不顯著，但是複合感染病株則與對照達極顯著之差異。複合專染病株中兩病毒之濃度均較單獨感染時提高，提高的程度以CMV較顯著。利用單隻桃蚜可將複合病株中之CMV或BICMV以單獨或複合之狀態傳至健株，其總和傳播率較之以單獨惑染病株作傳染源時提高甚多，以上證據顯示兩病毒間發生相乘效應(synergism)導至長豇豆發生皺葉嵌紋病。
A virus disease of asparagus bean with severe rugose mosaic symptoms was found widespread in the field. Two viruses were isolated from the diseased plants using tobacco and kidney bean as filter hosts. The host reactions, physical properties in crude sap, particle morphology and transmission characteristics indicated that one of the two viruses was cucumber mosaic virus (CMV) while the other blackeye cowpea mosaic virus (BICMV). The BICMV isolate was also proved to be serologically identical with BICMV from the United States by SDS-immunodiffusion tests. When asparagus beans were inoculated by mechanical means or by aphid vectors with both CMV and
BICMV, simultaneously or in sequences, the leaves above the inoculated ones would show the rugose mosaic symptom, and the pods would develop abnormally and become necrotic at a late stage. However, when the test plants inoculated with virus singly, CMV caused only leaf mottling whereas B1CMV caused vein banding mosaic. Neither could cause abnormal pods. In the greenhouse, no differences were found in the growth, yield and quality of asparagus beans between plants inoculated with CMV or B1CMV singly and those of the healthy controls. On the contrary, the growth; yield and quality of plants doubly infected by CMV and B1CMV differed significantly from those of the healthy ones. The concentration of either virus in doubly infected plants was higher than in singly infected ones. Aphid transmission studies showed
that CMV and BICMV could be transmitted from doubly infected plants by one single aphid to cause single or dual infections in asparagus beans. The percentage of transmission from using doubly infected plants as virus sources was about two times greater than from using singly infected sources. These results suggest a synergistic effect between CMV and BICMV in asparagus beans.
|Appears in Collections:||植物病理組|
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