|Title:||南黃薊馬(Thrips palmi Karny)之室內飼育方法||Other Titles:||Rearing Method of Southern Yellow Thrips, Thrips palmi Karny, in Laboratory||Authors:||王清玲
|Keywords:||南黃薊馬;室內飼育方法;Thrips palmi Karny;Laboratory-rearing method||Issue Date:||Oct-1986||Publisher:||植病保護學會||Journal Volume:||28||Journal Issue:||4||Start page/Pages:||407-411||Source:||植物保護學會會刊||Abstract:||
南黃薊馬(Thrips palmi Karny)成蟲飼育於玻管(4.3 × 1.5 cm)中，管內置南瓜葉片(1.5 × 1.5 cm)以為其食物與產卵處所。管內放入面積約15 cm2之吸水紙卷並加水0.05ml以調節濕度。每1至3日需更換葉片。以暗視野解剖顯微鏡(10X)可清晰檢視產於葉片組織內之卵粒。孵化之幼蟲飼育於下墊濕紗布之南瓜葉片上。老熟幼蟲再度置於上述大小之玻管中，內置含水0.2 ml之吸水紙卷(10 × 1.5 cm)以為化蛹場所。以此法飼育，在25℃，12L：12D之環境下，有61.5%的卵可發育為成蟲，自卵至成蟲之平均發育期為13.73日。羽化率為66.6%，但孵化率、幼蟲存活率、化蛹率均在95%以上。室內飼育之蟲體其體長、頭寬、翅長等均與野外採集者無顯著差異。
Adult southern yellow thrips (Thrips palmi Karny) were reared in a glass vial (4.3 x 1.5 cm) with a piece of pumpkin leaf (1,5 x 1.5 cm) as food and oviposition substrate. Paper towel about 15 cm2 with 0.05 ml of. water was added at the bottom of the vial to adjust the humidity. The leaf should be renewed every 1 to 3 days. Eggs in leaf tissue looked transparent and easily to be found under light contrast anascope (10 X). The first instar larvae were reared on pumpkin leaf placed on wet cheesecloth, while the mature larvae were again reared in the vials (4.3 x 1.5 cm) in which a roll of paper towel (10 x 1.5 cm) with 0.2 ml of water were placed in the vial as pupating site. By using this rearing method, some 61.5 percent of the eggs. could develop to adults. Developing, from egg to adult took 13.73 days in average. Although the emerging rate from pupa to adult was recorded as 66.6%, the hatching rate of eggs, the survival rate of larvae, and the pupating rate were all over 95%. Body sizes of laboratory-reared and field-captured adults were compared and no significant difference was observed.
|Appears in Collections:||應用動物組|
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