|Title:||The breeding of all Male Asparagus Plants by Means of Anther Culture||Other Titles:||蘆筍利用花藥培養之全雄育種||Authors:||Tsay, H.S.
|Keywords:||Taiwan Asparagus Research;蘆筍||Issue Date:||Oct-1986||Publisher:||台南區農業改良場||Start page/Pages:||13-22||Source:||民國72~74年度台灣蘆筍試驗研究報告||Abstract:||
The purpose of this experiment was to obtain homozygous male and female asparagus plants through the techniques of anther culture. Special efforts were made A) to clarify the breeding efficiency (number of green plants produced per 100 cultured anthers) of the individual plants, the effects of the different differentiation media, and the chromosome distribution of anther-derived plants, B) to distinguish the different ploidy through cytological squash method and phenotypic characterization, and C) to develop a chromosome doubling method for anther-derived haploid seedings. The results are summarized as follows:
1) breeding efficiency varied among individual plants and it was highest (14.6%) for U.C 500 W-20 among the 80 plants tested. This variation was probable due to genetic diversity. The organ regeneration process of anther-derived callus was well-established. Cytological study of root tips of the anther-derived plants revealed marked variation in chromosome number including haploid (8.2%), diploid (60.6%), tetraploid (24.3%), triploid (5.3%) and mixoploid (1.3%).
2) Plant type, internode length, leaf color, plyllocladia length and flower size were compared among haploid, diploid and tetraploid plants. It was found that early phenotypic evalution, both in vitro and in vivo, were highly potentiated.
3) Chromosome doubling of the haploid plants was achieved by several colchicine treatment methods. Obtaining diploid plants through callus of haploid shoot tip culture was more reliable than through colchicine treatment in terms of the frequency and stability of chromosome doubling.
(1)花藥癒合組織誘導及綠芽化率，單株間差異很大，因而造成品種及單株間育種效率之不等，差異可能與單株具不同之因子型有關，在80受測雄株中，以UC 500 W-20之育種效率14.6%最高。癒合組織所得植株之染色體分布，單倍體8.2%，雙倍體60.6%，四倍體24.3%，三倍體5.3%，混雜體最少僅1.3%。
|Appears in Collections:||生物技術組|
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