https://scholars.tari.gov.tw/handle/123456789/6669
DC Field | Value | Language |
---|---|---|
dc.contributor | Agricultural Chemistry Division, Agricultural Research Institute | en |
dc.contributor | College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University | en |
dc.contributor.author | Ming-Hui Chang | en_US |
dc.contributor.author | Chiu-Chung Young | en_US |
dc.contributor.author | Shiuan-Yuh Chien | en_US |
dc.contributor.author | A.B. Arun | en_US |
dc.creator | Ming-Hui Chang | en |
dc.creator | Chiu-Chung Young | en |
dc.creator | Shiuan-Yuh Chien | en |
dc.creator | A.B. Arun | en |
dc.date | 2008 | - |
dc.date.accessioned | 2012-01-31T03:26:26Z | - |
dc.date.accessioned | 2020-05-12T01:57:27Z | - |
dc.date.available | 2012-01-31T03:26:26Z | - |
dc.date.available | 2020-05-12T01:57:27Z | - |
dc.date.issued | 2008-06 | - |
dc.identifier.issn | 1590-4261 | - |
dc.identifier.uri | https://scholars.tari.gov.tw/handle/123456789/6669 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007/BF03175322 | - |
dc.description.abstract | The Aspergillus ficuum phytase gene phyA was overexpressed in Pichia pastoris X33 after replacing Buffered glycerol-complex medium (BMGY medium) using 1% (v/v) glycerol with fresh Buffered methanol-complex medium (BMMY medium) using 1% (v/v) methanol (on daily basis) as carbon sources. The phytase activity increased evidently with the induction time, and reached 200 U mL(-1) after 9 days of induction. We examined the possibility of employing thus obtained phytase to recover phosphorus from the fermented liquid of rice bran. When the 0.1 M sodium acetate buffer was replaced with de-ionised water (pH 5.5 +/- 0.1) as an enzyme reaction solution, there was an increase in the phosphorus recovery with respect to time and reached 1.31% after 24 h incubation contributing to 81% release of inorganic P from the rice bran phytate. Studies on hydrolysis of rice bran phytate by the addition of different concentrations of phytase ranging from 0-200 U mL(-1) produced through the recombinant yeast shows no significant effect in the rate of phytate hydrolysis at enzyme activities of 200, 100, 50 U mL(-1). However, rate of hydrolysis varied significantly at 20, 5, 0 U mL(-1) enzyme concentrations. | en_US |
dc.format.extent | 117 bytes | - |
dc.format.mimetype | text/html | - |
dc.language | en_US | en |
dc.language.iso | en_US | en_US |
dc.publisher | Springer | en_US |
dc.relation.ispartof | Annals of Microbiology | en_US |
dc.subject | phytase | en_US |
dc.subject | Pichia pastoris X33 | en_US |
dc.subject | phosphorus | en_US |
dc.subject | rice bran | en_US |
dc.subject | SDS-PAGE | en_US |
dc.title | Expression of recombinant Pichia pastoris X33 phytase for dephosphorylation of rice bran fermented liquid | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1007/BF03175322 | - |
dc.identifier.isi | 000257733800010 | - |
dc.relation.journalvolume | 58 | en_US |
dc.relation.journalissue | 2 | en_US |
dc.relation.pages | 233-238 | en_US |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.languageiso639-1 | en_US | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Applied Microbiology Laboratory | - |
crisitem.author.parentorg | Agricultural Chemistry Division | - |
Appears in Collections: | SCI期刊 |
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