|Title:||利用ISSR分子標誌鑑定咖啡種原親緣關係之研究||Other Titles:||Application of ISSR Molecular Marker Technology on Identification of Relationship of Coffee (Coffea spp.) Germplasm||Authors:||張淑芬
|Issue Date:||Jun-2012||Publisher:||中華種苗學會||Journal Volume:||14||Journal Issue:||2||Start page/Pages:||17-30||Source:||植物種苗||Abstract:||
The Coffea spp. germplasm accessions planted at Chiayi Agricultural Experiment Station, Taiwan Agricultural Research Institute, Chiayi were used in this research. The inter-simple sequence repeat (ISSR) molecular marker technology was applied to study the genetic relationship of thirty four coffee accessions. A total of 100 UBC's ISSR primers were screened for their ability to produce strongly amplified products and 19 of them were selected positively. Out of 337 DNA bands amplified by these 19 primers, 249 were polymorphic (74%). In accessions of C. arabica, HAES6590 had 3 specific bands and L11, L23 and Caturra(Y) all had one specific band which could be used for identification of these accessions. A dendrogram based on the UPGMA cluster analysis was constructed. All tested coffee germplasm accessions were divided into two groups and the genetic similarity of distance was 0.58. One group included C. canephora and C. liberica and the other one was C. arabica. The genetic similarity among the 32 accessions of C. arabica ranged from 0.66 to 0.99. Caturra(Y) had the lowest 0.66 similarity with other C. arabica accessions, indicting that it had the farest genetic relationship to the rest of C. arabica. The other 31 accessions had more than 0.88 genetic similarity with each other.
|Appears in Collections:||園藝系|
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