稻穿根線蟲Hirschmanniella oryzae及新紀錄種H. mucronata (Nematoda: Pratylenchidae)在台灣稻田之分佈

Abstract

自2004年12月起，從台灣地區稻田植株的根組織中共分離22群Hirschmanniella oryzae (van Breda de Haan，1902) Luc & Goodey，1964和8群H. mucronata (Das，1960) Luc & Goodey，1963，其中後者爲台灣地區的新記錄種。H. oryzae分怖於全台各地，但H. mucronata僅侷限於台灣中部的彰化縣和雲林縣二地區。各群稻穿根線蟲之種類鑑定依據包括外觀形態、形態测量植(morphometrics)及核糖體DNA (ribosomal DNA，rDNA)片段（包括完整5.8S基因，部分18S和28S基因及內轉錄區間ITS-1和ITS-2）的序列相同度比對分析。本研究提供更多完整的形態測量值和SEM外觀形態方面資料，並首次在GenBank基因庫中登錄這二種稻穿根線蟲的rDNA片段代表性序列，大小分別爲1001bp和962bp，而其序列相同度爲84.6%。 Root samples collected from several rice fields in Taiwan yielded 22 populations of Hirschmanniella oryzae (van Breda de Haan, 1902) Luc & Goodey, 1964 and 8 populations of H. mucronata (Das, 1960) Luc & Goodey, 1963., and the latter was a new record for Taiwan. H. oryzae was more widely distributed than H. mucronata, which was confined to central part of Taiwan. Based on the external morphology, morphometrics and the sequences of ribosomal DNA (including complete 5.8S gene, partial 18S and 28S gene, and complete internal transcribed spacer ITS-l, ITS-2), each nematode population was confirmed to the species level. In this study, additional morphometric data and SEM figures for these two rice root nematode were also provided. The representative sequences of rDNA fragment for these two species had the size of 1001bp and 962bp respectively were submitted to GenBank.