台灣地區茶園鞘線蟲Hemicriconemoides kanayaensis和新記錄種 H. californianus (Nematoda: Criconematoidea, Criconematidae)之鑑定

Abstract

自2006年4月起，從七個台灣主要產茶專業區的根圈土壤中，以改良式柏門氏漏斗分離法，總計分離到9群鞘線蟲(Hemicriconemoides spp.)。各群鞘線蟲之種類鑑定以外觀形態、形態測量值(morphometrics)及核糖體DNA (ribosomal DNA, rDNA)片段（包括完整5.8S基因，ITS-1和ITS-2內轉錄區間及部分18S和28S基因）序列的比對分析，確定其中6群為H. kanayaensis Nakasono & Ichinoe，1961，3群爲H. californianus Pinochet & Raski，1975，而後者為首次於茶樹上發表之寄生線蟲種類，也是台灣地區的新記錄種。本研究提供二種鞘線蟲雌蟲和雄蟲完整的形態測量值；其中雌蟲包括c'、m、EP、p、VL及VW等共6項，雄蟲包括c'、spicule、EP、p、Tail及ABW等共6項，和SEM外觀形態方面的資料，並首次在GenBank中登錄H. kanayaensis和H. californianus的rDAN片段代表性序列，其序列大小分別為1043bp和1051bp，而二者序列相似度為87.4%。 Nine populations of Hemicriconemoides spp. has isolated by modified Baermann funnel method from the rhizosphere soil of tea plants among 7 major tea production areas in Taiwan since April. 2006. Comparisons of the morphology, morphometrics and ribosomal DNA fragment sequences (include full 5.8S gene, ITS-1, ITS-2 and partial 18S and 28S gene) has yielded 6 populations being H. kanayaensis Nakasono & Ichinoe, 1961 and 3 populations being H. californianus Pinochet & Raski, 1975. H. californianus was the first reported in Taiwan. In this study, additional morphometric data (including c', m, EP, P. VL and VW of female; c', spicule. EP, P. Tail and ABW of male) and SEM figures for these two sheath nematode were provided. The representative sequences of rDNA fragment of H. kanayaensis and H. californianus had the size of 1043 bp and 1051 bp, respectively, and were firstly submitted to GeuBank.