|Title:||台灣甘藷品種之ISSR DNA標誌變異研究||Other Titles:||Variation in ISSR DNA Markers among Varieties of Sweet Potato (Ipomoea batatas L.) Cultivated in Taiwan||Authors:||簡靖華
|Keywords:||甘藷;分子標誌;品種鑑定;Sweet potato;Molecular marker;Variety identification||Issue Date:||Aug-2009||Publisher:||台灣農學會||Journal Volume:||10||Journal Issue:||4||Start page/Pages:||255-271||Source:||台灣農學會報||Abstract:||
To evaluate the genetic diversity of sweet potato varieties collected in Taiwan, ISSR DNA markers were applied in this study. Eighty-one informative markers detecting differences among varieties were proposed after the screen of 100 primers. And 23 out of these markers with strong and stable bands were used to establish DNA fingerprints of each variety and to evaluate the genetic diversity among germplasm. Results of genetic similarity analysis revealed the genetic distance among varieties ranged from 12.5% to 94.4%, and the average distance was 54.9%. The new varieties selected from polycross populations were distiribtued in different groups of cluster analysis indicating the wide diversity among the varieties. However, large genetic distance between varieties developed in Taiwan and germplasm introduced from China was observed both in cluster analysis and principal component coordinate analysis suggesting the potential uses of China germplasm as breeding parents in the future. In addition, an effective scheme, employing 6 primers to amplify 14 polymorphic markers, for variety identification was proposed. To detect somatic variation that resulted from vegetative propagation, individual plants from 2 varieties (TNG 66 and TAY 1) for tube production and 2 (TNG 71 and TAY 2) for leaf production were tested with ISSR markers. About 0.94%~1.86% of ISSR DNA variation for each variety was detected from 2640~3510 surveyed markers. The DNA fingerprints developed in this study would provide information for variety identification, clonal propagation, and genetic improvement of sweet potato.
|Appears in Collections:||農藝系|
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