Optimization of cultivation conditions for fungichromin production from Streptomyces padanus PMS-702

Jiun-Yan Wu; Jenn-Wen Huang; Hsin-Der Shih; Wei-Chen Lin; Yung-Chuan Liu

doi:10.1016/j.jcice.2007.11.006

Optimization of cultivation conditions for fungichromin production from Streptomyces padanus PMS-702

Resource

Journal of the Chinese Institute of Chemical Engineers 39(1): 67-73

Journal

Journal of the Chinese Institute of Chemical Engineers

Journal Volume

39

Journal Issue

1

Pages

67-73

Date Issued

2008-01

Date

2008-01

Author(s)

Jiun-Yan Wu

Jenn-Wen Huang

Hsin-Der Shih

Wei-Chen Lin

Yung-Chuan Liu

DOI

10.1016/j.jcice.2007.11.006

URI

https://scholars.tari.gov.tw/handle/123456789/3935

Abstract

A novel strain, Streptomyces padanus PMS-702, was employed to produce fungichromin (FC, a polyene macrolide antibiotic) in a shake flask cultivation. In comparing the effects of various carbon and nitrogen sources on PMS-702 cultivation, it was found that glucose and soybean meal medium yielded the highest FC within 2 days. Factors such as medium composition, cultivation temperatures, and initial pH were optimized for FC production with response surface methodology (RSM). The optimal cultural condition obtained is as follows: glucose 11.2 g/L, soybean meal 11.2 g/L, CaCO3 0.46 g/L, temperature 31.7 °C, and an initial pH 5.5. Under these conditions, FC production reached 112 mg/L, about an increase of 2.86 times, as compared to results under basic conditions.

Subjects

Streptomyces padanus PMS-702

Fungichromin

Response surface methodology

Polyene macrolide antibiotic

Optimization

Publisher

Elsevier

Type

journal article

File(s)

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