利用質體參考物質以及多重聚合酶連鎖反應建構基改馬鈴薯之檢測與定量方法

Other Title
Development of Detection and Quantification Methods for Transgenic Potato Using Multiplex PCR and Plasmidic Reference Material
Resource
台灣農業研究: 65(1): 18-30
Journal of Taiwan Agricultural Research: 65(1): 18-30
Journal
台灣農業研究
Journal Volume
65
Journal Issue
1
Pages
18-30
Date Issued
2016-03-03
Date
2016-03-03
Author(s)
馮昱維
吳明哲
王熙宇
Yu-Wei Feng
Min-Tze Wu
Shi-Yu Wang
DOI
URI
Abstract
基改作物一般無法以外觀型態加以區分，而透過以聚合酶連鎖反應 (polymerase chain reaction; PCR) 所發展的各項檢測技術，則可明確進行篩選與區別。 The objectives of this research were to establish a new quantification method and a fast multitarget PCR method for genetically modified potato event, EH92-527-1. A plasmidic reference material (PRM), pGEM-EH92, was constructed and used as a calibrant to quantify content of EH92-527- 1.
Subjects
基因改造
馬鈴薯
即時聚合酶鏈鎖反應
質體參考物質
Genetically modified
Potato
Real-time PCR
Plasmidic reference material
Publisher
農業試驗所
Type
journal article
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