https://scholars.tari.gov.tw/handle/123456789/14095| Title: | 利用質體參考物質以及多重聚合酶連鎖反應建構基改馬鈴薯之檢測與定量方法 | Other Titles: | Development of Detection and Quantification Methods for Transgenic Potato Using Multiplex PCR and Plasmidic Reference Material | Authors: | 杜元凱 馮昱維 吳明哲 王熙宇 陳述 陳涵葳 Yuan-Kai Tu Yu-Wei Feng Min-Tze Wu Shi-Yu Wang Shu Chen Han-Wei Chen |
Keywords: | 基因改造;馬鈴薯;即時聚合酶鏈鎖反應;質體參考物質;Genetically modified;Potato;Real-time PCR;Plasmidic reference material | Issue Date: | 3-Mar-2016 | Publisher: | 農業試驗所 | Journal Volume: | 65 | Journal Issue: | 1 | Start page/Pages: | 18-30 | Source: | 台灣農業研究 | Abstract: | 基改作物一般無法以外觀型態加以區分，而透過以聚合酶連鎖反應 (polymerase chain reaction; PCR) 所發展的各項檢測技術，則可明確進行篩選與區別。 The objectives of this research were to establish a new quantification method and a fast multitarget PCR method for genetically modified potato event, EH92-527-1. A plasmidic reference material (PRM), pGEM-EH92, was constructed and used as a calibrant to quantify content of EH92-527- 1. |
URI: | https://scholars.tari.gov.tw/handle/123456789/14095 | ISSN: | 0022-4847 | DOI: | 10.6156/JTAR/2016.06501.03 |
| Appears in Collections: | 1.台灣農業研究(1950～迄今) |
| File | Size | Format | |
|---|---|---|---|
| 65-1-3.pdf | 3.82 MB | Adobe PDF | View/Open |
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