High-level Tryptophan Accumulation in Seeds of Transgenic Rice and Its Limited Effects on Agronomic Traits and Seed Metabolitc Profile

Kyo Wakasa; Hisakazu Hasegawa; Hiroshi Nemoto; Fumio Matsuda; Haruna Miyazawa; Yuzuru Tozawa; Keiko Morino; Akira Komatsu; Tetsuya Yamada; Teruhiko Terakawa; Hisashi Miyagawa

High-level Tryptophan Accumulation in Seeds of Transgenic Rice and Its Limited Effects on Agronomic Traits and Seed Metabolitc Profile

Journal

Ecological and Environmental Biosafety of Transgenic Plants

Part Of

農業試驗所特刊第126號

Pages

163-190

ISBN

978-986-00-7734-6

986-00-7734-7

Date Issued

2006-12

Date

2006-12

Author(s)

Kyo Wakasa

Hisakazu Hasegawa

Hiroshi Nemoto

Fumio Matsuda

Haruna Miyazawa

Yuzuru Tozawa

Keiko Morino

Akira Komatsu

Tetsuya Yamada

Teruhiko Terakawa

Hisashi Miyagawa

URI

https://scholars.tari.gov.tw/handle/123456789/1544

Abstract

Metabolic manipulation of plants to improve their nutritional quality is an important goal of plant biotechnology. Expression in rice (Oryza sativa L.) of a transgene (OASA1D) encoding a feedback-insensitive a subunit of rice anthranilate synthase results in the accumulation of tryptophan (Trp) in calli and leaves. It is shown here that the amount of free Trp in the seeds of such plants is increased by about two orders of magnitude compared with that in the seeds of wild-type plants. The total Trp content in the seeds of the transgenic plants was also increased. Two homozygous lines, HWI and HWS, of OASA1D transgenic rice were generated for High-level Tryptophan Accumulation in Seeds of Transgenic Rice characterization of agronomic traits and aromatic metabolite profiling of seeds. The marked overproduction of Trp was stable in these lines under field conditions, although spikelet fertility and yield, as well as seed germination ability, were reduced compared with the wild type. These differences in agronomic traits were Small, however, in HWS. In spite of the high Trp content in the seeds of the HW lines, metabolic profiling revealed no substantial changes in the amounts of other phenolic compounds. The amount of indole acetic acid was increased about2- fold in the seeds of the transgenic lines. The establishment and characterization of these OASA1D transgenic lines have thus demonstrated the feasibility of increasing the Trp content in the seeds of rice (or of other crops) as a means of improving its nutritional value for human consumption or animal feed.

Event(s)

Proceedings of International Symposium Ecological and Environmental Biosafety of Transgenic Plants

Subjects

Amlno acids

Anthranilate Synthase

AS

Noncontainment greenhouse

IAA

Isolated field

5MT

OASA1D

Oryza sativa

Ecological and Environmental Biosafety of Transgenic Plants : proceedings of international symposium

Publisher

農業試驗所

Type

conference paper

File(s)

Name

publication_no126-16.pdf

Size

2.12 MB

Format

Adobe PDF

Checksum

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