Anti-inflammatory effects of Cynanchum taiwanianum rhizome aqueous extract in IL-1 beta beta-induced NRK-52E cells
Journal
Pharmaceutical Biology
Journal Volume
49
Journal Issue
4
Pages
437-444
Date Issued
2011-04
Author(s)
Hou-I Lee
Jia-Hsin Guo
Chih-Chung Wu
Mao-Chang Ti
Kuang-Ping Lan
Chun-Yi Hu
Chih-Yong Lo
Chi-Ching Yang
Yaw-Feng Lin
Tzu-Ching Wang
Abstract
Objective: In the present study, we investigated the anti-inflammatory effect of C. taiwanianum T. Yamaza rhizome aqueous extract (CTAE).
Materials and methods: The present study investigated the anti-inflammatory effect of CTAE using IL-1 beta beta-induced NRK-52E cells. Production of NO and PGE(2) by ELISA, the mRNA and protein expression of iNOS and COX-2, phosphorylation of I kappa kappa B alpha alpha, and activation of NF-kappa kappa B by RT-PCR and western blotting were determined.
Results: The CTAE significantly (P < 0.05) inhibited NO and PGE(2) production (decreased by 46.1% and 51%, respectively), and also significantly (P < 0.05) attenuated protein and mRNA expression of iNOS and COX-2 (decreased by 90% and 55% for iNOS and by 72% and 74%% for COX-2, respectively) in IL-1 beta beta-induced NRK-52E cells, in a dose-dependent manner, without obvious cytotoxic effects. Furthermore, the CTAE suppressed the NF-kappa kappa B nuclear translocation, in terms of inhibition of I kappa kappa B alpha alpha phosphorylation.
Discussion and conclusion: Our results provided evidence for its folkloric uses and suggest that the anti-inflammatory activities of CTAE may result from the inhibition of inflammatory mediators, such as NO and PGE(2), and an upstream suppression of a NF-kappa kappa B-dependent mechanism, might be involved.
Subjects
Cynanchum taiwanianum T. Yamaza
anti-inflammatory effect
cyclooxygenase-2
nitric oxide synthase
nuclear factor-kappa B
Publisher
Tylor & Francis Ltd.
Type
journal article