https://scholars.tari.gov.tw/handle/123456789/19392
Title: | Anti-inflammatory effects of Cynanchum taiwanianum rhizome aqueous extract in IL-1 beta beta-induced NRK-52E cells | Authors: | Hou-I Lee Jia-Hsin Guo Chih-Chung Wu Mao-Chang Ti Kuang-Ping Lan Chun-Yi Hu Chih-Yong Lo Chi-Ching Yang Yaw-Feng Lin Tzu-Ching Wang |
Keywords: | Cynanchum taiwanianum T. Yamaza;anti-inflammatory effect;cyclooxygenase-2;nitric oxide synthase;nuclear factor-kappa B | Issue Date: | Apr-2011 | Publisher: | Tylor & Francis Ltd. | Journal Volume: | 49 | Journal Issue: | 4 | Start page/Pages: | 437-444 | Source: | Pharmaceutical Biology | Abstract: | Objective: In the present study, we investigated the anti-inflammatory effect of C. taiwanianum T. Yamaza rhizome aqueous extract (CTAE). Materials and methods: The present study investigated the anti-inflammatory effect of CTAE using IL-1 beta beta-induced NRK-52E cells. Production of NO and PGE(2) by ELISA, the mRNA and protein expression of iNOS and COX-2, phosphorylation of I kappa kappa B alpha alpha, and activation of NF-kappa kappa B by RT-PCR and western blotting were determined. Results: The CTAE significantly (P < 0.05) inhibited NO and PGE(2) production (decreased by 46.1% and 51%, respectively), and also significantly (P < 0.05) attenuated protein and mRNA expression of iNOS and COX-2 (decreased by 90% and 55% for iNOS and by 72% and 74%% for COX-2, respectively) in IL-1 beta beta-induced NRK-52E cells, in a dose-dependent manner, without obvious cytotoxic effects. Furthermore, the CTAE suppressed the NF-kappa kappa B nuclear translocation, in terms of inhibition of I kappa kappa B alpha alpha phosphorylation. Discussion and conclusion: Our results provided evidence for its folkloric uses and suggest that the anti-inflammatory activities of CTAE may result from the inhibition of inflammatory mediators, such as NO and PGE(2), and an upstream suppression of a NF-kappa kappa B-dependent mechanism, might be involved. |
URI: | https://www.tandfonline.com/doi/full/10.3109/13880209.2010.517538 https://scholars.tari.gov.tw/handle/123456789/19392 |
ISSN: | 1388-0209 | DOI: | 10.3109/13880209.2010.517538 |
Appears in Collections: | SCI期刊 |
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