以即時反轉錄-聚合酶連鎖反應技術鑑別與檢測甜椒之馬鈴薯Y病毒及番椒斑駁病毒
Other Title
Development of Real-Time Reverse Transcription-Polymerase Chain Reaction to Identify and Detect Potato Virus Y and Pepper Mottle Virus in Sweet Pepper
Journal
台灣農業研究
Journal Volume
71
Journal Issue
1
Pages
11-18
Date Issued
2022-03
Author(s)
Abstract
本研究利用real-time reverse transcription-polymerase chain reaction (RT-PCR) 核酸增幅技術，首次建立可鑑定和區分馬鈴薯Y 病毒 (potato virus Y) 及番椒斑駁病毒 (pepper mottle virus) 之方法。結果顯示本核酸增幅技術，可同時偵測甜椒罹染馬鈴薯Y 病毒及番椒斑駁病毒，與其他茄科病毒及健康之植物無反應，對於單一或是複合感染皆可鑑定，由結果顯示此核酸增幅技術具有專一性佳及靈敏度高等優點，且可用於定量病毒在罹病組織內之濃度，將可應用於田間番椒罹染此2 種病毒之監測。
The real-time reverse transcription-polymerase chain reaction (RT-PCR) method was, for the first, developed to identify and differentiate potato virus Y and pepper mottle virus in sweet pepper. The results showed that this nucleic acid amplification technology can simultaneously detect both potato virus Y and pepper mottle virus. No amplification was obtained from other Solanaceae viruses and healthy plants. The method can be used to identify single or multiple infections. The results indicated that this quantitative RT-PCR has the advantages of good specificity and high sensitivity. The technology will be applied to assess field surveillance for sweet pepper.
Subjects
馬鈴薯y病毒
番椒斑駁病毒
偵測
Real-time RT-PCR 增幅技術
potato virus y
pepper mottle virus
detection
real-time rt-pcr
Publisher
農業試驗所
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
71-1-2.pdf
Size
2.01 MB
Format
Adobe PDF
Checksum
(MD5):fa2f20ce175003f1760084e0086fc5e3