稻熱病菌對水銀離子耐性之研究
Other Title
Studies on the Drug Resistance of Piricularia Oryzae against Mercuric Chloride
Journal
農業研究
Journal Volume
13
Journal Issue
3
Start Page
44
End Page
50
Date Issued
1964-09-06
Date
1964-09-06
Author(s)
簡錦忠
C.C. Chien
Abstract
本試驗係於實驗室內探討兩種不同生理小種之稻熱病病原菌，由於連續施用水銀劑後是否可能產生變異性耐藥菌種而舉行，其結果簡述如下：
1. 致病力強之稻熱病菌生理小種，對 HgCl2 之自然抵抗性為0.050mM，致病力弱之生理小種為0.082mM。
2. 供試兩生理小種對水銀離子顯示不規則之耐性增強。培養至第六代達0.375mM為最高耐性。此後再連續培養四代，均未有增加耐性之趨勢。
3. 供試耐藥菌（0.114 mM）之兩種生理小種，連續培養於無藥劑之基本培養基內，其對水銀離子耐性之增加一致保持在0.20 mM，似可認本菌對水銀離子具有永續耐性。
4. 根據處理前與處理後之菌種接種於16種稻品種上所呈現病斑之差異，測定其病原性之結果，耐藥菌之病原性均強於連續培養後之正常菌，與處理前母菌之病原性比較，致病力強之生理小種耐藥菌與母菌完全相同，連續培養之正常菌且有退化現象。致病力弱之生理小種耐藥菌及正常菌均比母菌為強。
In order to determine the drug resistance of Piricularia oryzae, the rice blast fungus, against mercuric chloride (HgCl2), two different physiologic races of P. oryzae were tested in this experiment. The results are summarized as follows:
(1) The natural drug resistance of OS-45S, a strongly virulent race is at 0.05 mM; while 1C-32S, a weakly virulent race, at 0.082 mM.
(2) When the two different races of P. oryzae was cultured on the potato dextrose agar medium containing different concentrations of HgCl2 for 6 stages (one stage is formed of 2 weeks culture). The fungus acquired the highest degree of drug resistance at 0.375 mM.
(3) Even if the drug resistant fungus at 0.114 mM is cultured on the normal medium containing no mercuric chloride for 10 stages (one stage is formed of 2 weeks culture), its resistance is not lost.
(4) Comparing the disease reactions of 16 differential rice varieties to the drug resistant cultures with to normal 10-stage cultures (successive cultivation on normal medium containing no mercuric chloride) of these two different races, the drug resistant cultures of both races are stronger in infectivity than normal 10-stage cultures of them. While compared with mother cultures, the drug resistant culture of OS-45S race showed the same virulence as its mother culture, but the normal 10-stage culture became weaker in virulence. However, the drug resistant culture and normal 10-stage culture of 1C-32S race were more virulent than their mother culture.
Publisher
農業試驗所
Type
journal article
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