應用試管培養方式評估根腐線蟲在山藥內之繁殖能力

Other Title
Evaluation of Reproduction of Pratylenchus coffeae on Yam (Dioscorea spp.) in vitro Culture
Journal
台灣農業研究
Journal Volume
59
Journal Issue
1
Pages
42-48
Date Issued
2010-03-04
Date
2010-03-04
Author(s)
賴素玉
許淑麗
楊宏仁
Su-Yu Lai
Shu-Li Hsu
Hong-Ren Yang
DOI
URI
Abstract
本研究利用試管培養方式評估根腐線蟲(Pratylenchus coffeae)在不同山藥品系之繁殖能力。將無菌培養於胡蘿蔔塊根癒傷組織之根腐線蟲母蟲，分別接種於台農5號、中埔018及杉林067三種山藥品系之試管中，置於25℃定溫箱每日12小時光照處理15週後，測定試管內山藥根系及培養基上線蟲之數量。 Pratylenchus coffeae is a major pathogenic nematode causing severe yield losses of yam (Dioscorea spp.) in Taiwan. The objective of this study was to evaluate reproduction of P. coffeae on yam in vitro culture. Female P. coffeae cultured on carrot callus tissues were inoculated on yam roots, cv. Tainong No.5, Chungpu 018, and Shanlin 067 in test tubes.
Subjects
山藥
根腐線蟲
繁殖能力
試管培養
Yam
Dioscorea spp.
Nematode
Pratylenchus c offeae
Reproduction
In vitro culture
Publisher
農業試驗所
Type
journal article
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