細胞分裂素與透氣性容器封口對丹參組培苗增殖與玻璃質化之影響
Other Title
Influence of Cytokinin and Ventilating Container Closure on Shoot Proliferation and Hyperhydricity of In Vitro Salvia miltiorriza Culture
Journal
台灣農業研究
Journal Volume
54
Journal Issue
2
Pages
93-102
Date Issued
2005-06-03
Date
2005-06-03
Author(s)
Abstract
本研究藉由改善細胞分裂素與培養容器透氣性，探討丹參(Salvia miltiorriza Bunge)組培苗大量繁殖及抑制玻璃質化苗形成之方法。將丹參莖節培養於含有1mg/l苄氨基嘌呤(benzyladenine, BA)與0.1mg/l奈乙酸(α-naphthaleneacetic acid, NAA)之全量MS (Murashige & Skoog)無基鹽類基本培養基中，可成功地誘導芽體形成及建立初代無菌丹參培養苗。
In vitro shoot multiplication of Salvia miltiorriza Bunge using nodal segment explants has been investigated in this study. Aseptic shoots were established on a full-strength MS medium containing 1 mg/l benzyladenine (BA) and 0.1 mg/l α-naphthaleneacetic acid (NAA) for shoot multiplication. In the cytokinin for shoot multiplication experiment, among the concentrations of BA (0.05, 0.1 mg/l) and kinetin (0.25, 0.5 mg/l) tested, the highest shoot multiplication (6.5 shoots per explant) was achieved in the medium containing 0.25 mg/l kinetin after 6 weeks of culture.
Subjects
丹參
藥用植物
微體繁殖
玻璃質化
Salvia miltiorrhiza
Medicinal herbs
Micropropagation
Hyperhydricity
Ventilating container closure
Publisher
農業試驗所
Type
journal article
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