基因轉殖作物品種檢測技術之開發－利用35S啓動子分子標誌檢測轉基因玉米及大豆

Abstract

本研究係分別以轉抗殺草劑基因之大豆以及轉抗蟲基因之玉米作為模式植物，建立基因轉殖植物在DNA層次之初步檢測分析。研究中基因轉殖與非基因轉殖之玉米及大豆共十七個樣品，經比較不同DNA萃取成本、DNA純度以及聚合酵素連鎖反應結果，以Wizard(上標 TM) Genomic DNA Purincation Kit萃取結果較佳。 Biotechnology has enabled the modification of crops in a very precise way thereby improving productivity and yield. Till 2003 about 78 transgenic crops were through safety evaluated and already approved. Since that, the problem for discriminating between transgenic crops and non- transgenic crops in the market was posed. In this study, we used herbicide resistance transgenic soybean and Bacilnus tumufacisos (Bt) corn as model plants for setup the DNA level detection of transgenic crops.