https://scholars.tari.gov.tw/handle/123456789/12276
Title: | 基因鎗轉殖台農67號水稻影響因子研究 | Other Titles: | Optimization Parameters of Particle Bombardment for Tainung 67 Rice (Oryza sativa L.) | Authors: | 夏奇鈮 顏信沐 曾東海 卓緯玄 王強生 Chi-Ni Hsia Hsin-Muh Yen Tung-Hai Tseng Woei-Shyuan Jwo Chang-Sheng Wang |
Keywords: | 水稻;基因鎗;轉殖;Rice (Oryza sativa L.);Particle gun;Transformation | Issue Date: | 1-Sep-2001 | Publisher: | 農業試驗所 | Journal Volume: | 50 | Journal Issue: | 3 | Start page/Pages: | 1-11 | Source: | 中華農業研究 | Abstract: | 本研究以本省最普遍栽培之水稻品種一台農67號之成熟種子為材料,利用基因鎗(Biolistic PDS1000/He device, Bio-Rad)進行基因轉殖。將成熟種子去殼後培養於添加2, 4-D之培養基中誘導胚性癒合組織增生,並以此材料測試影響基因鎗轉殖效率之各項因子,轉殖效率評估以GUS基因之暫時表現率為依據。 The objective of this study was to establish a genetic transformation system using biolistic bombardment (Biolistic PDS 1000/He device, Bio-Rad) for the most popular rice cultivar 'Tainung 67' in Taiwan. Embryogenic calli were induced from mature seeds on the medium containing 2, 4-D and they were used as material for bombardment. Transformation efficiency was evaluated by the transient expression of /β-glucuronidase gene. |
URI: | https://scholars.tari.gov.tw/handle/123456789/12276 | ISSN: | 0376-477X | DOI: | 10.29951/JARC.200109.0001 |
Appears in Collections: | 1.台灣農業研究(1950~迄今) |
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50-3-1.pdf | 1.93 MB | Adobe PDF | View/Open |
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