甘藷種原試管中保存技術之研究 (I)碳素源對培殖體生長之影響

Abstract

甘藷無病毒苗之莖段培養於含MS基本無機鹽類，0.4 mg/l thiamine-HC1，100 mg/1 myo-inositol，1 mg/1 IAA，6％ sucrose及0.8％ Difco agar之改良式MS固體培養基，可使培殖體生長旺盛，且移植後生長勢強，對加速種原繁殖極具功效。為延長種原保存的期限，於上述改良式MS培養基中，以3％ glucose取代6％ sucrose，則可抑制培殖體生長，延長繼代培養（subculture）週期達6個月，其培殖體若再移植培養於3％ sucrose之改良式MS培養基中，即可迅速恢復其生長勢。Experiments were conducted to study the influence of sugar sources on the in vitro growth and maintenance of sweet potato cuttings for the purpose of efficient germpiasm preservation. Stem segments of virus-free sweet potato plants were successfully cultured on a medium containing Murashige and Skoog (MS) inorganic salts supplemented with 0.4mg/l thiamine-HC1, 100mg/l myo-inositol, 1mg/l IAA, 6% sucrose and 0.8% Difco agar.