大蒜潛隱病毒在臺灣之發生及其生物特性
Other Title
Occurrence of garlic latent virus in Taiwan and its biological characterization
Resource
中華農業研究 40(3): 333-345
Journal of Agricultural Research of China 40(3): 333-345
Journal
中華農業研究
Journal Volume
40
Journal Issue
3
Pages
333-345
Date Issued
1991-09-10
Date
1991-09-10
Author(s)
鄧汀欽
蔡淑娓
蔡財旺
T.C. Deng
S.W. Tsai
T.W. Tsai
Abstract
從大陸進口的北蒜中分離出來的一個病毒單株，經鑑定是大蒜潛隱病毒(garlic latent virus, GLV)。其寄主範圍包括大蒜、洋葱、葱、韭等葱科作物，但呈無病徵系統性感染，唯不感染韭葱；其他寄主反應包括：紅藜、Chenopodium quinoa、及番杏接種葉的局部病斑，豌豆的局部潛隱感染，蠶豆的系統性壞疽等。但對供試的千日紅、孤挺花、百合、水仙、菸草（五種）、酸漿等皆不感染。經試驗GLV之耐熱性爲55-60℃，耐稀釋性爲10^(-3)-10^(-4)，耐保存性是3-4天。以C. quinoa接種葉的局部病斑爲材料進行病毒純化，經硫酸絕等密度平衡離心，所得病毒試料之紫外光吸收光譜，最高值在258nm，最低值在243nm，其比值爲1.084；260nm的吸收值與280nm的吸收值之比是1.25。Garlic latent virus (GLV) was isolated from plants of ”northern garlic”, which seed- bulbs were imported from mainland China in 1989. It caused symptomless infection on garlic, onion, shallot, Welsh onion, Chinese leek, and pea, local lesions on Chenopodium amaranticolor, C. quinoa, and Tetragonia expansa, or systemic necrosis on broad bean, but did not infect leek, narcissus, lily, belldonna lily, tobacco (5 species), Gornphrena globosa, and Physalis floridana in host range test. The virus was propagated in Chinese leek and assayed in C. amaranticolor; thermal inactivation point (TIP) is 55-60℃, dilution end point (DEP) is 10^(-3)-10^(-4), and longevity in vitro is 3-4 days. Purified preparation was obtained from GLV inoculated leaves of C. quinoa by cesium sulfate isopycnic centrifugation. The ultraviolet absorption spectrum of GLV is typical of a nucleoprotein, with maximum at 258 nm, minium at 243 nm, Amax/Amin ratio is 1.084, and A260/A280 ratio is 1.25.
Subjects
蔥科作物
大蒜潛隱病毒
寄主反應
抗血清
發生
Allium
garlic latent virus
GLV
carlavirus
host
antiserum
occurrence
Publisher
農業試驗所
Type
journal article
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