薑花組織培養之大量繁殖
Other Title
In Vitro Propagation of Zingiber Zerumbet Smith
Journal
中華農業研究
Journal Volume
40
Journal Issue
2
Pages
171-177
Date Issued
1991-06-06
Date
1991-06-06
Author(s)
許家言
葉常青
蔡新聲
J.Y. Hsu
C.C. Yeh
H.S. Tsay
Abstract
利用組織培養技術，進行薑花大量繁殖的研究，以期在短時間內獲得較多的栽培苗供推廣是本試驗之目的。結果顯示，液體培養的效果比固體培養好，保留頂芽處理的分蘗芽數較切除頂芽者為多，基本培養基則以全量MS（Murashige and Skoog，1962）基本鹽類配合4 mg／1 BA所得的分蘗芽數最多，在培養六星期後每一個芽可繁殖4.78個芽體，若以液體培養配合保留頂芽處理的繁殖法，試管苗在培養二星期後即可全部發根。利用本試驗推薦的方法繁殖薑花，一個芽可在一年中產生8×105株幼苗。
The purpose of this experiment was to develop effective in vitro method to mass-propagate ginger flower in a short period of time as required for commercial cultivation. Shoot tips (1 mm in size) were cultured on MS medium containing 2 mg/l of 6-benzyladenine, 0.05 mg/l of NAA, 40 mg/l of adenine sulfate, 170 mg/l of NaH2PO4•H2O and 3% of sucrose for 1 month. The adventitious buds produced from these cultures were used to evaluate the effect of topping and the composition of culture medium on the production of secondary adventitious buds.
Subjects
薑花
大量繁殖
Ginger flower
mass propagation
Publisher
農業試驗所
Type
journal article
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