The Microspore Development and Haploid Embryogenesis of Anther Culture with Five Nitrogen Doses to the Donor Tobacco Plants

Abstract

By growing N. tabacurn plants (Wisconsin 38) with different levels of nitrogen in basic Hoagland water solution, it was found that the amount of nitrogen supply can strongly influence the physiological status of the donor plants and thus the embryogenic ability of cultured anthers. Highest embryogenic ability was obtained when donor plants were grown in culture solution containing 15 mM nitrogen. 菸草以改良 Hoagfand 基本鹽類的水耕液培養，發現氮素不同量的供給能左右母體植物生長的好壞，進而影響到由此植物所取花葯培養單倍體的形成。花葯培養的結果顯示低濃度氮素量生長下的母體植物（尤其是不含氮素量處理區），其單倍體之形成較差。