Organ Regeneration from Anther Callus of Sweet Potato
Other Title
甘藷花葯癒傷組織器官之形成
Journal
中華農業研究
Journal Volume
31
Journal Issue
2
Pages
123-126
Date Issued
1982-06-04
Date
1982-06-04
Author(s)
H.S. Tsay
P.C. Lai
L.J. Chen
蔡新聲
賴本智
陳良築
Abstract
Callus was induced from anthers of Ipomoea batatas Poir. by culturing on Murashige and Skoog basic medium supplemented with 2 mg/l each of 2,4-D, IAA and kinetin or 2 mg/l each of NAA and kinetin. Embryoids or shoots could be regenerated directly from ABA-containing medium. A large number of roots could be induced by subculturing these embryoids to 1/2 strength of MS medium with 0.1 mg/l NAA, 0.5 mg/l kinetin and 7.5 mg/l adenine sulfate, a few buds were observed from the nodule-like tissue of the growing root after 2-4 months in the same medium.
含有 2,4-D, IAA 及 Kinetin 各 2 mg/l 或 NAA 及 Kinetin 各 2 mg/l 之 MS 基本鹽類培養基，可誘導甘藷花葯產生癒傷組織。含有 Abscisic acid 之 MS 基本鹽類培養基，可直接誘導癒傷組織形成擬胚體或芽體，將擬胚體繼代培養於含有 NAA 0.1 mg/l , kinetin 0 . 5 mg/l 及 adenine sulfate 7 . 5 mg/l 之 1/2 濃度 MS 基本鹽類培養基可誘導多量的根形成，繼代培養這些生長中的根經 2-4 個月後，可自根之瘤狀部形成少數芽體。
Publisher
農業試驗所
Type
journal article
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