|Title:||不同接種季節對水稻花藥培養的影響||Other Titles:||Effect of Inoculation Time during the Year on the Culture Efficiency of Rice Anthers||Authors:||葉常青
|Issue Date:||2-Sep-1987||Publisher:||農業試驗所||Journal Volume:||36||Journal Issue:||3||Start page/Pages:||247-257||Source:||中華農業研究||Abstract:||
Rice plants (cv Tainung 67) were transplanted year round and anthers were collected and inoculated in different months of the year in order to investigate the effect of environmental factors, especially temperature, on the efficiency of rice anther culture. Results showed that the rate of callus formation for anthers inoculated between April and December ranged between 27 and 42%. Anthers collected in February failed to produce any callus. No anthers were available for culture in January and March due to the influence of temperature of the development of rice plants. Low temperature under which the rice donor plants were grown affected callus formation more severely than did the high temperature. The reproductive growth of rice plant, especially the meiosis of pollen mother cells, was more sensitive to extreme temperature than vegetative growth. Histological study indicated that low temperature inhibited microspore formation and caused abnormal multiplication of tapetum layer cells. No relationship was established between the rates of callus formation and plantlet regeneration in anther culture for rice plants grown in different seasons. The influence of average air temperature on the callus differentiation ability was also not clear.
|Appears in Collections:||1.台灣農業研究(1950～迄今)|
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