不同接種季節對水稻花藥培養的影響
Other Title
Effect of Inoculation Time during the Year on the Culture Efficiency of Rice Anthers
Journal
中華農業研究
Journal Volume
36
Journal Issue
3
Pages
247-257
Date Issued
1987-09-02
Date
1987-09-02
Author(s)
葉常青
蔡新聲
C.C. Yeh
H.S. Tsay
Abstract
以週年栽培方式種植水稻臺農67號，探討水稻母株生育環境對花藥培養形成癒合組織及分化成植株的影響，結果發現4至12月份所接種的花藥癒合組織形成率約在27~42%之間;由於低溫造成水稻植株的生長遲滯，因而錯失了1月及3月份的資料，而在2月份低溫下產生的花藥，則完全無癒合組織的形成。以水稻母株生育環境的溫度而言，低溫較高溫對花藥癒合組織的形成能力有較為不利的影響。就水稻母株的生育過程而言，生殖生長期對溫度變化的反應比營養生長期敏感，尤其以花粉母細胞減數分裂期為最。經切片觀察，低溫會造成絨氈層細胞的異常增殖，及產生多核性巨大小孢子，而阻止小孢子發育。癒合組織的分化率並不隨著癒合組織形成率的高低而變化，亦無法找出其與外界溫度的關係。
Rice plants (cv Tainung 67) were transplanted year round and anthers were collected and inoculated in different months of the year in order to investigate the effect of environmental factors, especially temperature, on the efficiency of rice anther culture. Results showed that the rate of callus formation for anthers inoculated between April and December ranged between 27 and 42%. Anthers collected in February failed to produce any callus. No anthers were available for culture in January and March due to the influence of temperature of the development of rice plants. Low temperature under which the rice donor plants were grown affected callus formation more severely than did the high temperature. The reproductive growth of rice plant, especially the meiosis of pollen mother cells, was more sensitive to extreme temperature than vegetative growth. Histological study indicated that low temperature inhibited microspore formation and caused abnormal multiplication of tapetum layer cells. No relationship was established between the rates of callus formation and plantlet regeneration in anther culture for rice plants grown in different seasons. The influence of average air temperature on the callus differentiation ability was also not clear.
Publisher
農業試驗所
Type
journal article
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