微小昆蟲卵之染色觀察法
Other Title
Different Staining Methods for Eggs of Diminutive Insects
Journal
中華農業研究
Journal Volume
36
Journal Issue
3
Pages
331-334
Date Issued
1987-09-12
Date
1987-09-12
Author(s)
呂鳳嗚
Feng-Ming Lu
Abstract
多種產卵於葉肉組織內之害蟲如潛蠅類、薊馬類其產卵量之觀察不易，本文用1%酸性洋紅液及乳酸酚一酸性洋紅液染色後，可在解剖顯微鏡下清晰觀察雌蟲產卵量。前者無毒且價廉，對埋於葉肉組織（厚、薄葉類）之卵的染色效果很好，其卵呈暗紅色；但其需較長之處理時間。後者配方為酚：乳酸：蒸餾水：甘油以1 : 1 : 1 : 2 之比例配或250mL 之溶液，再加入0.25g 之酸性洋紅；其染色效果更加明顯，卵呈紫紅色。但因其中酚具毒性及腐蝕性，且價格較昂，當需短時間內得知結果時，可使用水浴加熱法，適用於少量調查或薄葉類染色時，若大量調查或厚葉類染色時，為恐加熱時間太長有欠安全，故改用浸泡法進行，個人可視情況加以選用。
Eggs of many leaf miners and thrips are laid in the plant tissues. We used two staining solutions in order to detect and count the eggs which are 1% aqueous acid fuchsin and lactopenol acid fuchsin. The preceding solution is cheaper and no poison but need long time to stain it. The other solution is more effective but costed higher and also poisoned to the human nervous system. There fore, we usually heat it in water bath for the rapid dipping of the thin leaf, and in immersion method of long period dipping for the thick leaf, it will decrease the damage of the toxic phenol. In conclusion, we select the proper staining method for the special purose. After treatment, the color of eggs became deep red or purple red that were easier to observe.
Publisher
農業試驗所
Type
journal article
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