甘藷病毒 SPV-A 及 SPV-N 消除法之研究
Other Title
Studies on Eradication of SPV-A and SPV-N Viruses from Infected Sweet Potato
Journal
中華農業研究
Journal Volume
31
Journal Issue
3
Pages
239-245
Date Issued
1982-09-10
Date
1982-09-10
Author(s)
廖嘉信
蔡新聲
盧英權
Chia-Hsin Liao
Hsin-Sheng Tsay
Ying-Chuan Lu
Abstract
試驗目的在研究去除甘藷SPV-A 及SPV-N 病毒的有效方法。經以不同的熱療法（heat therapy）及化學療法（chemotherapy）為前處理，配合莖頂培養，得知以恆溫（38℃）熱處理時，處理期間長短與獲得無病毒苗的百分率間有極顯著的正相關。變溫熱處理（38o / 32℃）則無顯著效果。莖頂的大小與獲得無病毒苗百分率間呈顯著的負相關，顯示SPV-A 及SPV-N 在莖頂呈不均等分佈，即近分生組織部分之病毒濃度較低或尚未分佈至莖頂。10 ppm 之kinetin 及l00 ppm 之2 - thiouracil 對SPV-A 及SPV-N 病毒的去除具有效果。
This study was conducted to test the effects of thermotherapy and chemotherapy on the culture of shoot tips in vitro for producing virus-free seedlings from virus infected sweet potato. The duration of constant heat treatment (38°C) of sweet potato plants was positively correlated with the percentage of virus-free seedlings obtained (r=0.8749**). On the other hand, no correlation between these two factors was found at day/night temperatures of 38/32°C (r=0.6053). The negative correlation between the size of excised shoot tips and the percentage of virus-free seedlings obtained revealed uneven distribution of virus SPV-A and SPV-N in the shoot tips. Lowest concentration of virus at the position closest to the meristem was suggested. Culture medium containing 10 ppm of kinetin or 100 ppm of 2-thiouracil exhibited the inhibitory effect on virus mulitplication.
Publisher
農業試驗所
Type
journal article
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