Heat Induced Germination of Basidiospores of Agaricus Bisporus (Lge.) Sing

Abstract

Induced spore germination of A. bisporus as affected by heat treatment was studied. The optimum temperature for the stimulation of basidiospore germina-tion was 45°C. The time required for the treatment to obtain better germination was also studied. For spore germination 10 to 50 minutes of heat treatment was considered effective. The optimum temperature for incubation of the heat-treated basidiospores was 28°C. The highest percentage of spore germination thus obtained was 25 per cent. 1. 本試驗應用溫度處理洋菇孢子，探求處理孢子發芽最適合之溫度與時間，俾能提高洋菇孢子之發芽率，進而應用於單孢分離，選種培養與育種工作。 2. 洋菇孢子發芽之溫度自40℃到55℃不等，而以45℃ 為最適溫度。 3. 洋菇孢子在45℃之溫度下處理5分鐘到90分鐘不等，均可刺激其發芽，其中以10分鐘到50分鐘為最佳，而且具有同樣效果。 4. 最適洋菇孢子發芽之培養溫度為28℃，最低極限為18℃，最高極限為34℃。 5. 洋菇孢子經45℃處理30分鐘後培養於28℃下，可將其發芽率提高達百分之二十五。