水稻培養花葯褐變與癒傷組織形成之關係
Other Title
The Relationship between Anther Browning and Callus Formation in Rice Anther Culture
Journal
中華農業研究
Journal Volume
29
Journal Issue
2
Pages
137-146
Date Issued
1980-06-08
Date
1980-06-08
Author(s)
賴本智
蔡新聲
紀迺淳
Pen-Chih Lai
Hsin-Sheng Tsay
Nai-Chwen Chi
Abstract
臺農67 號水稻花葯以六種不同消毒方法處理後培養，結果發現消毒液濃度愈高，消毒時問愈長，會促使花葯愈早褐變，因而造成癒傷組織形成能力的降低；秈稉稻雜交F1 之花藥，培養後褐變的早晚，各雜交組合問亦呈褐變早者癒傷組織形成能力低，褐變晚者癒傷組織形成能力高的趨勢。培養花藹主第4-6 週褐變者癒傷組識形成率最高。培養花葯癒傷組織的產生，約在培養後第21 至72 天之間，早於21 天或晚於72 天，形成癒傷組織機會極少，絕大多數癒傷組織在培養後36-51 天間形成。花葯早期褐變所導致癒傷組織形成能力之低下。可能係酚類物質氧化所生之qulnone 產生毒害，不利於小胞子發育形成癒傷組織所致。
Anthers of Oryza sativa cv. Tainung No. 67 containing microspores at uninucleate stage were sterilized with six different methods and were cultured at 27°C for 90 days to investigate the relationship between the formation of callus and browning of anthers. It was found that anther browning caused by sterilization reagents severely affected the ability of callus formation. In general, higher concentration or longer duration of sterilization accelerated the browning of cultured anthers and reduced the ability of callus induction.
The culture of F1 hybrid anthers from six cross combinations of japonica x indica rices also showed the association between earlier anther browning and lower callus formation ability. The anthers turned brown in the later period, e. g., four to six weeks after culture, were shown to possess a greater potential for callus formation than those browned earlier.
The callus formation started at 21 days and ended at 72 days after culture. the highest pecentage of callus induction from anthers was observed from 36 to 51 days after culture. The low callus formation ability caused by anther browning at early stage was probably due -to the production of quinones which are toxic to the microspores.
Publisher
農業試驗所
Type
journal article
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