異戊烯轉移酶(ipt)基因轉殖青花菜花粉活性及其相近菜種雜交之研究

Other Title
Pollen Viability and Cross Ability of Isopentenyl Transferase (ipt)-Gene Transformed Broccoli (Brassica oleracea var. italica) and Its Relatives
Resource
台灣農業研究 59(4): 261-274
Journal of Taiwan Agricultural Research 59(4): 261-274
Journal
台灣農業研究
Journal Volume
59
Journal Issue
4
Pages
261-274
Date Issued
2010-12-05
Date
2010-12-05
Author(s)
丁柏瑜
張翔
高于雯
呂秀英
陳榮芳
吳明哲
陳烈夫 
Po-Yu Ting
Hsiang Chang
Yu-Wen Kao
Hsiu-Ying Lu
Long-Fang O. Chen
Min-Tze Wu
Lit-Fu Chan 
DOI
URI
Abstract
基因流佈 (gene flow) 是基因轉殖作物生態風險評估中一個重要指標，而花粉活性與雜交稔實率是影響作物異交潛力的主要因子。本試驗利用異戊烯轉移酶(isopentenyltransferase, ipt) 基因轉殖青花菜 (Brassica oleracea var. italica) 103 品系與非轉殖青花菜對照組 (104 品系及品種‘綠王’)，和另外5 種相近菜種 [白花芥藍 (B. oleracea var. alboglabra)-‘富悅’、黃花芥藍 (B. oleracea var. alboglabra)-‘白格林’、花椰菜 (B. oleracea var. botrytis)-‘麗雪’、小白菜 (B. rapa var. chinensis)、小油菜 (B. rapa var. japonica)] 等材料，透過分析花粉活性與雜交率對青花菜異交潛力進行評估，提供基因轉殖青花菜商業栽培需隔離與建立共存之參考。 Gene flow is an important index for environmental biosafety assessments. Pollens are potential carriers for genetically modified DNA to other plants. For phanerogams, pollen viability and cross-compatibility are key factors for successful outcross hybridization. In this study, broccoli (Brassica oleracea var. italica) transformed with the ipt gene, designated as line 103 was used to investigate its potential outcross by pollen viability test and pollination with non-transgenic broccolis (line 104 and cv. 'Green King') and closely 5 related varieties, such as Chinese kales (B. oleracea var. alboglabra) 'Fu Yue' (white flower) and 'Bai Ge Lin' (yellow flower), cauliflower (B. oleracea var. botrytis) 'Li Syue', Pakchoi (B. rapa var. chinensis), and edible rape (B. rapa).
Subjects
芥藍
基因流佈
雜交率
花期重疊
轉基因青花菜
Chinese kale
Gene flow
Hybridization percentage
Overlapped flowering days
Transgenic broccoli
Publisher
農業試驗所
Type
journal article
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