培養基鹽類配方、活性碳與蔬果添加物對台灣白及無菌播種之影響

Abstract

本研究藉由15種不同鹽類配方Kundson (Kundson 1946)、VW (Vacin & Went 1949)、全量及半量之MS (Murashige & Skoog 1962）以及花寶1號(HYPONeX 7-6-19)皆添加08% Bacto agar與3％蔗糖為基礎培養基，配合01％活性碳，以及不同含量之香蕉或馬鈴薯的添加，共4種試驗進行台灣白及黃熟果莢無菌播種，測試上述因子對台灣白及種子發芽與促進芽體生長發育之影響。 This study was conduced to determine effect to basal salts, active charcoal and vegetable/fruit homogenates on seed germination and seedling development of Bletilla formosana (Hayata) Schltr in in vitro culture. Bacto agar (0.8%) and sucrose (3%) were used to prepare the five culture media, using reported basal formulations of Knudson (Knudson 1946), VW (Vacin & Went 1949), MS (Murashige & Skoog 1962), 1/2 MS, or HYPONeX 7-6-19. Results showed that seeds of B. formosana were all germinated on these basal salt media after incubation at (25±1℃) for 8 days.